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Literature summary extracted from

  • Jurkowski, T.P.; Meusburger, M.; Phalke, S.; Helm, M.; Nellen, W.; Reuter, G.; Jeltsch, A.
    Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism (2008), RNA, 14, 1663-1670.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.204 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.204 C292A about 3fold reduction in RNA binding affinity. Significant residual activity Homo sapiens
2.1.1.204 C79A about 3fold reduction in RNA binding affinity. No detectable in vitro methylation activity Homo sapiens
2.1.1.204 E119A mutant binds stronger to RNA than wild-type. No detectable in vitro methylation activity Homo sapiens
2.1.1.204 R160A no detectable in vitro methylation activity Homo sapiens
2.1.1.204 R162A no detectable in vitro methylation activity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.204 Mg2+ supports native folding of the substrate tRNA Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.204 Drosophila melanogaster
-
-
-
2.1.1.204 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.204
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNA in vitro transcribed tRNAAsp is methylated by DNMT2, albeit at reduced efficiency. Human, mouse, and Dictyostelium tRNA all are substrates for human DNMT2. C79, E119, R160 and R162 are essential for the catalytic mechanism of DNMT2 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.204 Dnmt2
-
 Drosophila melanogaster
2.1.1.204 Dnmt2
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.204 7.5
-
 assay at Homo sapiens