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Literature summary extracted from

  • Goda, S.K.; Rashidi, F.A.; Fakharo, A.A.; Al-Obaidli, A.
    Functional overexpression and purification of a codon optimized synthetic glucarpidase (carboxypeptidase G2) in Escherichia coli (2009), Protein J., 28, 435-442.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.11 overexpression of a codon-optimized gene in Escherichia coli, vector pET28a, the enzyme is expressed to about 60% of the total host protein and the purification of the recombinant His-tagged protein could be achieved in a single step by Ni2+ charged column chromatography Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.11 EDTA
-
 Pseudomonas sp.
3.4.17.11 additional information Mg2+ inhibits the activity of the recombinant enzyme; Mn2+ inhibits the activity of the recombinant enzyme Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.11 additional information Mg2+ inhibits the activity of the recombinant enzyme Pseudomonas sp.
3.4.17.11 additional information Mn2+ inhibits the activity of the recombinant enzyme Pseudomonas sp.
3.4.17.11 Zn2+ zinc dependant Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.11 41000
-
 SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.11 Pseudomonas sp. P06621
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.11 as His-tagged protein in a single step by Ni2+ charged column chromatography Pseudomonas sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.17.11 Refolding is initiated by rapid 25fold dilution of the denatured protein into refolding buffer (100 mM Tris-HCl, pH 8.5, containing 0.1 mM EDTA and 0.5 M arginine) Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.11 methotrexate + H2O
-
 Pseudomonas sp. 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.11 carboxypeptidase G2
-
 Pseudomonas sp.
3.4.17.11 CPG2
-
 Pseudomonas sp.
3.4.17.11 glucarpidase
-
 Pseudomonas sp.