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Literature summary extracted from

  • Mi, Q.; Yang, J.; Ye, F.; Gan, Z.; Wu, C.; Niu, X.; Zou, C.; Zhang, K.
    Cloning and overexpression of Pochonia chlamydosporia chitinase gene pcchi44, a potential virulence factor in infection against nematodes (2010), Process Biochem., 45, 810-814.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14
-
 Pochonia chlamydosporia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 acetazolamide complete inhibition Pochonia chlamydosporia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.00965
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 5.5, 42°C Pochonia chlamydosporia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Pochonia chlamydosporia
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 44000
-
 x * 44000, SDS-PAGE Pochonia chlamydosporia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pochonia chlamydosporia B5L0W0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 glycoprotein sequence contains two putative N-glycosylation sites Pochonia chlamydosporia
3.2.1.14 proteolytic modification the putative primary signal peptidase cleavage site is between residues A22 and T23, and the second processing step is downstream to R35 and A36 Pochonia chlamydosporia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 25.85
-
 pH 5.5, 42°C Pochonia chlamydosporia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Pochonia chlamydosporia 4-methylumbelliferol + N,N',N''-triacetylchitotriose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 44000, SDS-PAGE Pochonia chlamydosporia

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chi44
-
 Pochonia chlamydosporia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Pochonia chlamydosporia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
 Pochonia chlamydosporia

General Information

EC Number General Information Comment Organism
3.2.1.14 physiological function chitinase can damage eggs of both the root-knot nematode Meloidogyne incognita and the insect Bombyx mori Pochonia chlamydosporia