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Literature summary extracted from

  • Willger, S.D.; Ernst, J.F.; Alspaugh, J.A.; Lengeler, K.B.
    Characterization of the PMT gene family in Cryptococcus neoformans (2009), PLoS ONE, 4, e6321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.109 PCR-amplification of reference strain open reading frames cloned into standard cloning vectors, expressed in fungus Cryptococcus neoformans var. neoformans
2.4.1.109 PCR-amplification of reference strain open reading frames cloned into standard cloning vectors, expressed in fungus Cryptococcus neoformans var. grubii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.109 additional information disruption after amino acid D439 results in pmt1A lacking mutants are viable but show distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, more susceptible to SDS medium, enlarged cells subject to spontaneous lysis, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, pmt1A mutant is susceptible to sorbitol (2.5 M) and to 0.1% SDS, reduced survival upon macrophage attack Cryptococcus neoformans var. grubii
2.4.1.109 additional information disruption after amino acid E468 results in pmt2A lacking mutants, not viable, one wild-type allele is necessary for growth, after sporulation no haploid pmt2 lacking mutants are found, Pmt2 is essential but an intact Pmt2 gene lacking the other two isoforms is not sufficient for viability, may be due to interaction with the other isoforms Cryptococcus neoformans var. grubii
2.4.1.109 additional information disruption after amino acid K461 results in pmt4A lacking mutants, viable but with distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, poor growth on high salt medium, abnormal septum formation, enlarged cells subject to spontaneous lysis, multi-cell aggregate formation based on defects in cell separation, delayed melanin production, slightly reduced capsule size, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, points to necessary interaction with the Pmt2 isoform or essential combined target, the pmt4A mutant is susceptible to sorbitol (2 M), reduced survival upon macrophage attack Cryptococcus neoformans var. grubii
2.4.1.109 additional information disruption after amino acid N508 results in pmt2D lacking mutants, not viable, one wild-type allele is necessary for growth, after sporulation no haploid pmt2 lacking mutants are found, Pmt2 is essential but an intact Pmt2 gene lacking the other two isoforms is not sufficient for viability may be due to interaction with the other isoforms Cryptococcus neoformans var. neoformans
2.4.1.109 additional information disruption after amino acid Q412 results in pmt4D lacking mutants, aviable but with distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, poor growth on high salt medium, abnormal septum formation, enlarged cells subject to spontaneous lysis, multi-cell aggregate formation based on defects in cell separation, delayed melanin production, slightly reduced capsule size, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, points to necessary interaction with the Pmt2 isoform or essential combined target, the pmt4D strain is not inhibited by sorbitol but is inhibited by SDS, bilateral crossing of two pmt4D mutant strains delays mating reaction with reduced filament formation, less aerial hyphae, irregular shape and thickness of filaments with swollen distal tips Cryptococcus neoformans var. neoformans
2.4.1.109 additional information disruption after amino acid W315 results in pmt1D lacking mutants are viable but show distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, more susceptible to SDS medium, enlarged cells subject to spontaneous lysis, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, the pmt1D mutant grows poorly in sorbitol, is unaffected by SDS, no defects in mating and crossing are observed Cryptococcus neoformans var. neoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.109 additional information cell wall destabilizing agents Congo red, caffeine, and calcofluor white have no effect on the growth of any pmt mutants Cryptococcus neoformans var. grubii
2.4.1.109 additional information cell wall destabilizing agents Congo red, caffeine, and calcofluor white have no effect on the growth of any pmt mutants; cell wall destabilizing agents Congo red, caffeine, and calcofluor white have no effect on the growth of any pmt mutants Cryptococcus neoformans var. neoformans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.109 endoplasmic reticulum
-
 Cryptococcus neoformans var. neoformans 5783
-
2.4.1.109 endoplasmic reticulum
-
 Cryptococcus neoformans var. grubii 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.109 additional information at 0.5 M KCl pmt4 mutant grows slower than wild-type, at 0.7 M growth inhibition Cryptococcus neoformans var. neoformans
2.4.1.109 additional information at 0.5 M KCl pmt4 mutant grows slower than wild-type, at 0.7 M growth inhibition Cryptococcus neoformans var. grubii
2.4.1.109 additional information at 1 M MaCl growth inhibition of mutant pmt1 but to a lesser extent than mutant pmt4 Cryptococcus neoformans var. grubii
2.4.1.109 additional information at 1 M NaCl growth inhibition of mutant pmt1 but to a lesser extent than mutant pmt4 Cryptococcus neoformans var. neoformans
2.4.1.109 additional information at 1 M NaCl growth inhibition of mutant pmt4 Cryptococcus neoformans var. neoformans
2.4.1.109 additional information at 1 M NaCl growth inhibition of mutant pmt4 Cryptococcus neoformans var. grubii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + protein Cryptococcus neoformans var. neoformans
-
 dolichyl phosphate + O-D-mannosylprotein
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein Cryptococcus neoformans var. grubii
-
 dolichyl phosphate + O-D-mannosylprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Cryptococcus neoformans var. grubii
-
 serotype A, wild-type strain H99, human fungal pathogen, strain used for pathogenesis experiments
-
2.4.1.109 Cryptococcus neoformans var. neoformans Q5KAF1 serotype D, wild-type strain JEC21 and JEC21, human fungal pathogen, strain used for pathogenesis experiments
-
2.4.1.109 Cryptococcus neoformans var. neoformans Q5KHK5 serotype D, wild-type strain JEC21 and JEC21, human fungal pathogen, strain used for pathogenesis experiments
-
2.4.1.109 Cryptococcus neoformans var. neoformans Q5KIZ1 serotype D, wild-type strain JEC21 and JEC21, human fungal pathogen, strain used for pathogenesis experiments
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.109 gel purification of disruption mutants Cryptococcus neoformans var. neoformans
2.4.1.109 gel purification of disruption mutants Cryptococcus neoformans var. grubii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + protein
-
 Cryptococcus neoformans var. neoformans dolichyl phosphate + O-D-mannosylprotein
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein
-
 Cryptococcus neoformans var. grubii dolichyl phosphate + O-D-mannosylprotein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 Pmt1A
-
 Cryptococcus neoformans var. grubii
2.4.1.109 Pmt1D
-
 Cryptococcus neoformans var. neoformans
2.4.1.109 Pmt2A
-
 Cryptococcus neoformans var. grubii
2.4.1.109 Pmt2D
-
 Cryptococcus neoformans var. neoformans
2.4.1.109 Pmt4A
-
 Cryptococcus neoformans var. grubii
2.4.1.109 Pmt4D
-
 Cryptococcus neoformans var. neoformans
2.4.1.109 protein O-mannosyltransferase
-
 Cryptococcus neoformans var. neoformans
2.4.1.109 protein O-mannosyltransferase
-
 Cryptococcus neoformans var. grubii

Expression

EC Number Organism Comment Expression
2.4.1.109 Cryptococcus neoformans var. neoformans no change in expression of either isoform upon growth in nutrient rich medium at 30 and 37°C, with additional salt stress or under capsule-inducing conditions, expression is not unregulated due to deficiency in one other isoform additional information
2.4.1.109 Cryptococcus neoformans var. grubii no change in expression of either isoform upon growth in nutrient rich medium at 30 and 37°C, with additional salt stress or under capsule-inducing conditions, expression is not unregulated due to deficiency in one other isoform additional information

General Information

EC Number General Information Comment Organism
2.4.1.109 malfunction virulence of the human fungal pathogen causing meningoencephalitis depends on extracellular factors including the O-glycosylation of proteins Cryptococcus neoformans var. neoformans
2.4.1.109 malfunction virulence of the human fungal pathogen causing meningoencephalitis depends on extracellular factors including the O-glycosylation of proteins Cryptococcus neoformans var. grubii
2.4.1.109 physiological function O-glycosylation of proteins Cryptococcus neoformans var. neoformans
2.4.1.109 physiological function O-glycosylation of proteins Cryptococcus neoformans var. grubii