Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sanchez-Cach, L.A.; Ortiz-Garcia, M.M.; Minero-Garcia, Y.; Munoz-Sanchez, J.A.; Hernandez-Sotomayor, S.T.; Suarez-Solis, V.M.; De Los Santos-Briones, C.
    Isolation of cDNA encoding the catalytic site of phosphatidylinositol-specific phospholipase C from Coffea arabica L.: Recombinant expression and peptide purification (2008), Plant Signal. Behav., 3, 913-916.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.11 into the pET11d vector, expressed as a histidine-tagged protein in Escherichia coli BL21 cells Coffea arabica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.11 membrane
-
 Catharanthus roseus 16020
-
3.1.4.11 membrane
-
 Capsicum chinense 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.11 27000
-
 x * 27000, SDS-PAGE or Western blot analysis Coffea arabica
3.1.4.11 30000
-
 x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense
3.1.4.11 32000
-
 x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense
3.1.4.11 34700
-
 sequence analysis Coffea arabica
3.1.4.11 44000
-
 x * 44000, Western blot analysis Catharanthus roseus
3.1.4.11 44000
-
 x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Capsicum chinense
-
-
-
3.1.4.11 Catharanthus roseus
-
-
-
3.1.4.11 Coffea arabica B2LSE7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11 by Ni-NTA chromatography Coffea arabica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 additional information L2 cell line and aluminum-tolerant LAMt cell line Coffea arabica
-
3.1.4.11 root
-
 Coffea arabica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 phosphatidylinositol 4,5-bisphosphate + H2O
-
 Coffea arabica D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.11 ? x * 27000, SDS-PAGE or Western blot analysis Coffea arabica
3.1.4.11 ? x * 44000, Western blot analysis Catharanthus roseus
3.1.4.11 ? x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense

Synonyms

EC Number Synonyms Comment Organism
3.1.4.11 phosphatidylinositol-specific phospholipase C
-
 Catharanthus roseus
3.1.4.11 phosphatidylinositol-specific phospholipase C
-
 Coffea arabica
3.1.4.11 phosphatidylinositol-specific phospholipase C
-
 Capsicum chinense
3.1.4.11 PI-PLC
-
 Catharanthus roseus
3.1.4.11 PI-PLC
-
 Coffea arabica
3.1.4.11 PI-PLC
-
 Capsicum chinense

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.11 Coffea arabica sequence analysis
-
 5.31