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Literature summary extracted from

  • Huang, M.; Slewinski, T.L.; Baker, R.F.; Janick-Buckner, D.; Buckner, B.; Johal, G.S.; Braun, D.M.
    Camouflage patterning in maize leaves results from a defect in porphobilinogen deaminase (2009), Mol. Plant, 2, 773-789.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.61 Lambda ZAP Express system Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.61 additional information 4 insertion allels (m1-m4) of camouflage1 (cf1) mutant, mutant develops nonclonal, yellow-green sectors in leaves based on bundle sheath cell-specific death, yellow mutant regions show reduced levels of chlorophyll a + b, and total carotenoids, reduced photosynthesis and stomatal conductance compared to wild type and green regions of the mutant, constant light suppresses the cf1 sector formation, sectors only form during a limited time of leaf development, underlying is a decreased enzyme activity and increased levels of the substrate in green and yellow regions, yellow regions show an additional reduction in catalase activity Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.61 chloroplast fusion enzyme with yellow fluorescent protein (YFP), transformed in Vicia faba leaves for localization analysis Zea mays 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 4 porphobilinogen + H2O Zea mays
-
 hydroxymethylbilane + 4 NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Zea mays
-
 maize
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61 leaves of 8 week old field grown plants are ground in liquid nitrogen and extracted with 25 mM NaP buffer, pH 8.0, containing 1 mM EDTA, 5 mM DTT, and 0.5 mM phenylmethanesulfonylfluoride, centrifuged, supernatant taken for enzyme assay Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 fruit in developing ears Zea mays
-
2.5.1.61 leaf mature and etiolated leaves Zea mays
-
2.5.1.61 root seedling roots Zea mays
-
2.5.1.61 tassel in developing tassels Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
 cf1 mutant tissue shows 30% reduced activity (about 0.7 units/mg) compared to wild type (about 2.5 units/mg) in green and yellow leaf tissue, consistent with reduced enzyme abundance, 13fold substrate accumulation Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen + H2O
-
 Zea mays hydroxymethylbilane + 4 NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 PBGD
-
 Zea mays
2.5.1.61 porphobilinogen deaminase
-
 Zea mays

Expression

EC Number Organism Comment Expression
2.5.1.61 Zea mays cf1 mutation show strongly decreased expression compared to wild type down

General Information

EC Number General Information Comment Organism
2.5.1.61 malfunction camouflage1 (cf1) mutant develops nonclonal, yellow-green sectors in leaves based on bundle sheath cell-specific death, yellow mutant regions show reduced levels of chlorophyll a + b, and total carotenoids, reduced photosynthesis and stomatal conductance compared to wild type and green regions of the mutant, constant light suppresses the cf1 sector formation, sectors only form during a limited time of leaf development, underlying is a decreased enzyme activity and increased levels of the substrate in green and yellow regions, yellow regions show an additional reduction in catalase activity Zea mays
2.5.1.61 physiological function early step in chlorophyll and heme biosynthesis Zea mays