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Over-expression of phospholipase D isozymes down-regulates protein kinase CKII activity via proteasome-dependent CKIIbeta degradation in NIH3T3 cells

Yoon, S.H.; Min, d.o..S.; Bae, Y.S.; Mol. Cells 27, 299-305 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.4
overexpression of isozymes PLD1 and PLD2 in NIH3T3 cells
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
K758R
catalytically inactive mutant of PLD2
Mus musculus
3.1.4.4
K898R
catalytically inactive mutant of PLD1
Mus musculus
3.1.4.4
additional information
overexpression of phospholipase D isozymes, leads to downregulation of protein kinase CKII activity via proteasome-dependent CKIIbeta degradation in NIH3T3 cells. Co-localization of CKIIbeta and PLD isozymes in NIH3T3 cells
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
1-butanol
inhibits PLD hydrolytic activity
Mus musculus
3.1.4.4
additional information
no inhibition by 2-butanol
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
additional information
Mus musculus
PLD also performs transphosphatidylation using 1-butanol as phosphatidyl acceptor
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Mus musculus
-
isozymes PLD1 and PLD2
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
additional information
-
PLD transphosphatidylation activity, overview
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
PLD also performs transphosphatidylation using 1-butanol as phosphatidyl acceptor
709956
Mus musculus
?
-
-
-
-
3.1.4.4
additional information
the catalytic activity of PLD is not required for PLD-mediated CKII inhibition, possible inhibition mechanism, overview
709956
Mus musculus
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
overexpression of isozymes PLD1 and PLD2 in NIH3T3 cells
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
K758R
catalytically inactive mutant of PLD2
Mus musculus
3.1.4.4
K898R
catalytically inactive mutant of PLD1
Mus musculus
3.1.4.4
additional information
overexpression of phospholipase D isozymes, leads to downregulation of protein kinase CKII activity via proteasome-dependent CKIIbeta degradation in NIH3T3 cells. Co-localization of CKIIbeta and PLD isozymes in NIH3T3 cells
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
1-butanol
inhibits PLD hydrolytic activity
Mus musculus
3.1.4.4
additional information
no inhibition by 2-butanol
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
additional information
Mus musculus
PLD also performs transphosphatidylation using 1-butanol as phosphatidyl acceptor
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
additional information
-
PLD transphosphatidylation activity, overview
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
PLD also performs transphosphatidylation using 1-butanol as phosphatidyl acceptor
709956
Mus musculus
?
-
-
-
-
3.1.4.4
additional information
the catalytic activity of PLD is not required for PLD-mediated CKII inhibition, possible inhibition mechanism, overview
709956
Mus musculus
?
-
-
-
-