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Literature summary extracted from
- Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2 (2010), Microbiology, 156, 589-595.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.1.45 | expressed in Escherichia coli BL21(DE3) cells | Geobacillus thermodenitrificans |
| 1.14.14.8 | expressed in Escherichia coli | Geobacillus thermodenitrificans |
| 1.14.14.8 | expression in Escherichia coli | Geobacillus thermodenitrificans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.45 | Geobacillus thermodenitrificans | - |
- |
- |
| 1.5.1.45 | Geobacillus thermodenitrificans NG80-2 | - |
- |
- |
| 1.14.14.8 | Geobacillus thermodenitrificans | - |
- |
- |
| 1.14.14.8 | Geobacillus thermodenitrificans NG80-2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.45 | chelating Sepharose column chromatography | Geobacillus thermodenitrificans |
| 1.14.14.8 | using Ni-NTA chromatography | Geobacillus thermodenitrificans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.45 | 55.47 | - |
using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C | Geobacillus thermodenitrificans |
| 1.5.1.45 | 58.2 | - |
using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C | Geobacillus thermodenitrificans |
| 1.14.14.8 | 151.3 | - |
substrate anthranilate, pH 9.0, 60°C | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.45 | FAD + NADH + H+ | 95% activity with NADH relative to NADPH | Geobacillus thermodenitrificans | FADH2 + NAD+ | - |
? |  |
| 1.5.1.45 | FAD + NADPH + H+ | higher activity is obtained with FAD than with FMN using NADPH as the cofactor | Geobacillus thermodenitrificans | FADH2 + NADP+ | - |
? | |
| 1.5.1.45 | FMN + NADPH + H+ | higher activity is obtained with FAD than with FMN using NADPH as the cofactor | Geobacillus thermodenitrificans | FMNH2 + NADP+ | - |
? | |
| 1.5.1.45 | additional information | anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP | Geobacillus thermodenitrificans | ? | - |
? | |
| 1.14.14.8 | 2-hydroxyphenylacetate + FADH2 + O2 | 67.8% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
| 1.14.14.8 | 2-hydroxyphenylacetate + FADH2 + O2 | 67.8% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
| 1.14.14.8 | 2-hydroxyphenylacetate + FADH2 + O2 | 62.79% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
| 1.14.14.8 | 4-hydroxyphenylacetate + FADH2 + O2 | 42.7% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
| 1.14.14.8 | 4-hydroxyphenylacetate + FADH2 + O2 | 42.7% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
| 1.14.14.8 | 4-hydroxyphenylacetate + FADH2 + O2 | 42.7% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
| 1.14.14.8 | anthranilate + FADH2 + O2 | - |
Geobacillus thermodenitrificans | 3-hydroxyanthranilate + FAD + H2O | - |
? | |
| 1.14.14.8 | anthranilate + FADH2 + O2 | - |
Geobacillus thermodenitrificans NG80-2 | 3-hydroxyanthranilate + FAD + H2O | - |
? | |
| 1.14.14.8 | anthranilate + FADH2 + O2 | highest activity | Geobacillus thermodenitrificans | 3-hydroxyanthranilate + FAD | - |
? | |
| 1.14.14.8 | additional information | no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene | Geobacillus thermodenitrificans | ? | - |
? | |
| 1.14.14.8 | additional information | no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene | Geobacillus thermodenitrificans NG80-2 | ? | - |
? | |
| 1.14.14.8 | salicylate + FADH2 + O2 | 49.6% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
| 1.14.14.8 | salicylate + FADH2 + O2 | 49.6% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
| 1.14.14.8 | salicylate + FADH2 + O2 | 49% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.1.45 | FAD reductase | - |
Geobacillus thermodenitrificans |
| 1.5.1.45 | GTNG_3158 | - |
Geobacillus thermodenitrificans |
| 1.14.14.8 | FADH2-utilizing monooxygenase | - |
Geobacillus thermodenitrificans |
| 1.14.14.8 | GTNG_3160 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.8 | 60 | - |
- |
Geobacillus thermodenitrificans |
| 1.14.14.8 | 60 | - |
assay at, highest activity | Geobacillus thermodenitrificans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.8 | 25 | 70 | - |
Geobacillus thermodenitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.8 | 9 | - |
- |
Geobacillus thermodenitrificans |
| 1.14.14.8 | 9 | - |
assay at, highest activity | Geobacillus thermodenitrificans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.8 | 5 | 11 | - |
Geobacillus thermodenitrificans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.8 | FADH2 | - |
Geobacillus thermodenitrificans | |
| 1.14.14.8 | FADH2 | FADH2-utilizing monooxygenase, no reaction with FMNH2 | Geobacillus thermodenitrificans | |
| 1.14.14.8 | additional information | no cofactor: FMNH2 | Geobacillus thermodenitrificans |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.14.8 | Geobacillus thermodenitrificans | transcription level increases highly when anthranilate is used as the sole carbon source | up |
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Release 2023.1 (January 2023)
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