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Literature summary extracted from

  • Akiyama, T.; Jin, S.; Yoshida, M.; Hoshino, T.; Opassiri, R.; Ketudat Cairns, J.R.
    Expression of an endo-(1,3;1,4)-beta-glucanase in response to wounding, methyl jasmonate, abscisic acid and ethephon in rice seedlings (2009), J. Plant Physiol., 166, 1814-1825.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 gene OsEGL1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, functional expression od GST-tagged EGL1 in Escherichia coli Oryza sativa Japonica Group
3.2.1.73 gene OsEGL2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, functional expression of GST-tagged EGL2 in Escherichia coli Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Oryza sativa Q8S9P4 OsEGL2; cv. Yukihikari, gene OsEGL2
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3.2.1.73 Oryza sativa Japonica Group Q5UAW3 OsEGL1; cv. Yukihikari, gene OsEGL1
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 recombinant GST-tagged EGL1 from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine resin chromatography Oryza sativa Japonica Group
3.2.1.73 recombinant GST-tagged EGL2 from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine resin chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.73 root gene OsEGL1 is expressed in seedling, and young and adult plant roots Oryza sativa Japonica Group
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3.2.1.73 seedling
-
 Oryza sativa Japonica Group
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3.2.1.73 seedling
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-1,3/1,4-glucan + H2O best substrate is 1,3/1,4-beta-glucan from Hordeum vulgare, and almost equally active from Avena sativa Oryza sativa Japonica Group ?
-
 ?
3.2.1.73 beta-1,3/1,4-glucan + H2O best substrate is 1,3/1,4-beta-glucan from Hordeum vulgare, and almost equally active from Avena sativa Oryza sativa ?
-
 ?
3.2.1.73 lichenan + H2O a beta-1,3/1,4-glucan from Cetraria islandica, half as active as barley or oat beta-1,3/1,4-glucans Oryza sativa Japonica Group ?
-
 ?
3.2.1.73 lichenan + H2O a beta-1,3/1,4-glucan from Cetraria islandica, half as active as barley or oat beta-1,3/1,4-glucans Oryza sativa ?
-
 ?
3.2.1.73 additional information purified recombinant EGL1 protein hydrolyzes beta-1,3/1,4-glucans, but not beta-1,3/1,6-linked or beta-1,3-linked glucopolysaccharides, e.g. laminarin from Laminaria digitata or Eisenia bicyclis, or curdlan from Alcaligenes faecalis, nor carboxymethylcellulose Oryza sativa Japonica Group ?
-
 ?
3.2.1.73 additional information purified recombinant EGL2 protein hydrolyzes beta-1,3/1,4-glucans, but not beta-1,3/1,6-linked or beta-1,3-linked glucopolysaccharides, e.g. laminarin from Laminaria digitata or Eisenia bicyclis, or curdlan from Alcaligenes faecalis, nor carboxymethylcellulose Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 endo-(1,3;1,4)-beta-glucanase
-
 Oryza sativa Japonica Group
3.2.1.73 endo-(1,3;1,4)-beta-glucanase
-
 Oryza sativa

Expression

EC Number Organism Comment Expression
3.2.1.73 Oryza sativa the expression of endo-(1,3;1,4)-beta-glucanase is upregulated in response to wounding, methyl jasmonate, abscisic acid and ethephon in rice seedlings up
3.2.1.73 Oryza sativa Japonica Group the expression of endo-(1,3;1,4)-beta-glucanase is upregulated in response to wounding, methyl jasmonate, abscisic acid and ethephon in rice seedlings. The increase in the leaf elongation rate of rice seedlings treated under mechanical wounding is concomitant with an increase in OsEGL1 expression levels in seedling leaves, overview up