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Literature summary extracted from

  • Akif, M.; Georgiadis, D.; Mahajan, A.; Dive, V.; Sturrock, E.D.; Isaac, R.E.; Acharya, K.R.
    High-resolution crystal structures of Drosophila melanogaster angiotensin-converting enzyme in complex with novel inhibitors and antihypertensive drugs (2010), J. Mol. Biol., 400, 502-517.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.15.1 expression in Pichia pastoris Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.15.1 purified recombinant free AnCE and in complex with six antihypertensive drugs, captopril, enalaprilat, lisinopril, ramiprilat, trandolaprilat, perindoprilat, lisW-S, RXPA380, and RXP407, hanging drop vapour diffusion method at 16 °C, 0.002 ml of protein solution containing 10 mg/ml native AnCE in 5 mM HEPES, pH 7.5, 0.1 mM PMSF, and 0.01 mM zinc acetate, are mixed with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, and 1.5 M sodium citrate, for enzyme complexes, the crystals are mixed with inhibitor solution, X-ray diffraction structue determination and analysis at 1.85-2.10 A resolution Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 captopril
-
 Drosophila melanogaster
3.4.15.1 enalaprilat
-
 Drosophila melanogaster
3.4.15.1 lisinopril
-
 Drosophila melanogaster
3.4.15.1 lisW-S lisinopril-tryptophan S-enantiomer, a C-domain human sACE specific inhibitor and antihypertensive drug Drosophila melanogaster
3.4.15.1 perindoprilat
-
 Drosophila melanogaster
3.4.15.1 ramiprilat
-
 Drosophila melanogaster
3.4.15.1 RXP407 i.e. Ac-Asp-L-Phe(PO2CH2)-L-Ala-Ala-NH2, a human sACE domain-specific phosphinic peptidyl inhibitor and antihypertensive drug Drosophila melanogaster
3.4.15.1 RXPA380 i.e. (2S)-2-[([2-[(1R)-1-[((benzyloxy)carbonyl)amino]-2-(phenylethyl)(hydroxyl)-phosphinyl]cyclopentyl]carbonyl)amino]-3-(1H-indo-3-yl)-propionic acid (Cbz-PhePSI[P(O)(OH)CH]Pro-Trp-OH), a human sACE domain-specific phosphinic peptidyl inhibitor and antihypertensive drug Drosophila melanogaster
3.4.15.1 trandolaprilat
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.15.1 additional information Drosophila melanogaster AnCE is a single domain protein with ACE activity, an ACE homologue ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Drosophila melanogaster Q10714
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.1 recombinant enzyme from Pichia pastoris by hydrophobic interaction chromatography and gel filtration to homogeneity Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 additional information AnCE is a single domain protein with ACE activity, an ACE homologue Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.15.1 ACE
-
 Drosophila melanogaster
3.4.15.1 ANCE
-
 Drosophila melanogaster
3.4.15.1 angiotensin I-converting enzyme
-
 Drosophila melanogaster
3.4.15.1 angiotensin-converting enzyme
-
 Drosophila melanogaster