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Literature summary extracted from

  • Agnew, A.; Timson, D.
    Mechanistic studies on human N-acetylgalactosamine kinase (2009), J. Enzyme Inhib. Med. Chem., 25, 370-376.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.157 expression in Escherichia coli Homo sapiens
2.7.1.157 GALK2, functional expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.157 alpha-methylgalactopyranoside uncompetitive inhibition with respect to ATP; uncompetitive with respect to ATP, poor inhibitor Homo sapiens
2.7.1.157 N-acetyl-D-galactosamine substrate inhibition Homo sapiens
2.7.1.157 N-acetylgalactosamine substrate inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.157 0.014
-
 ATP recombinant enzyme Homo sapiens
2.7.1.157 0.014
-
 ATP pH 8.0, 37°C Homo sapiens
2.7.1.157 0.04
-
 N-acetyl-D-galactosamine recombinant enzyme Homo sapiens
2.7.1.157 0.04
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.157 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.157 ATP + N-acetyl-D-galactosamine Homo sapiens
-
 ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.157 Homo sapiens
-
-
-
2.7.1.157 Homo sapiens Q01415 isoform GALK2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.157 recombinant GALK2 from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.157 ATP + N-acetyl-alpha-D-galactosamine = ADP + N-acetyl-alpha-D-galactosamine 1-phosphate ordered ternary complex reaction mechanism, in which ATP binds first, overview. Ionizable residues play a role in substrate binding and catalysis Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.157 ATP + N-acetyl-alpha-D-galactosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.157 ATP + N-acetyl-D-galactosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.157 additional information ordered ternary complex mechanism in which ATP is the first substrate to bind Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.157 GALK2
-
 Homo sapiens
2.7.1.157 More GALK2 belongs to the GHMP family of enzymes Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.157 1
-
 ATP recombinant enzyme Homo sapiens
2.7.1.157 1
-
 N-acetyl-D-galactosamine recombinant enzyme Homo sapiens
2.7.1.157 1
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.157 additional information
-
 pH dependency of the reaction mechanism, overview Homo sapiens
2.7.1.157 7 8
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.157 ATP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.157 13
-
 N-acetylgalactosamine pH 8.0, 37°C Homo sapiens