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Literature summary extracted from

  • Yu, C.L.; Kale, Y.; Gopishetty, S.; Louie, T.M.; Subramanian, M.
    A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid (2008), J. Bacteriol., 190, 772-776.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.5.2 0.0037
-
 Caffeine pH 7.5, 35°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.5.2 20000
-
 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.
1.17.5.2 40000
-
 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.
1.17.5.2 90000
-
 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.
1.17.5.2 158000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.17.5.2 Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.5.2 1530
-
 pH 7.5, 35°C Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.17.5.2 -80°C, 5 mM potassium phosphate buffer pH 7.5, enzyme loses activity after a few days Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.5.2 caffeine + ubiquinone-0 preferred electron acceptor Pseudomonas sp. trimethyl uric acid + ubiquinol-0 the oxygen atom in trimethyl uric acid is derived exclusively from water ?
1.17.5.2 additional information enzyme is specific for caffeine and theobromine. No activity with xanthine, 3-methylxanthine, 7-methylxanthine, trimethyl uric acid, and 3,7-dimethyl uric acid Pseudomonas sp. ?
-
 ?
1.17.5.2 theobromine + ubiquinone-0 46% of the activity with caffeine Pseudomonas sp. ? + ubiquinol-0
-
 ?
1.17.5.2 theophylline + ubiquinone-0 0.6% of the activity with caffeine Pseudomonas sp. ? + ubiquinol-0
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.5.2 trimer 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.5.2 66
-
 or above, activity increases linearly from 25 to 66°C, which is the highest temperature allowed by the experimental conditions Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.5.2 42
-
 30 min, 77% residual activity Pseudomonas sp.
1.17.5.2 70
-
 30 min, 11% residual activity Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.5.2 0.167
-
 Caffeine pH 7.5, 35°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.5.2 7
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.5.2 6
-
 52% of maximum activity Pseudomonas sp.
1.17.5.2 8
-
 47% of maximum activity Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.5.2 additional information enzyme is not NAD(P)+-dependent Pseudomonas sp.
1.17.5.2 ubiquinone-0 preferred electron acceptor Pseudomonas sp.