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Literature summary extracted from

  • Al-Dabbagh, B.; Mengin-Lecreulx, D.; Bouhss, A.
    Purification and characterization of the bacterial UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase WecA (2008), J. Bacteriol., 190, 7141-7146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.33 3,6,9,12,15,18-hexaoxatriacontan-1-ol i.e. C12E6, optimal concentration is 90-200 mM Thermotoga maritima
2.7.8.33 Triton X-100 optimal concentration is 50-120 mM Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.33 cloned into pET2130, generating the plasmid pWTM8. The wecA gene product is expressed with an N-terminal polyhistidine tag, expression in Escherichia coli C43(DE3) Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.33 KCl 125 mM, about 50% of maximal activity Thermotoga maritima
2.7.8.33 Mg2+ 250 mM, no activity. 10 mM Mg2+ optimally activates the enzyme Thermotoga maritima
2.7.8.33 NaCl 200 mM, about 50% of maximal activity Thermotoga maritima
2.7.8.33 tunicamycin 11 nM, 50% inhibition Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.33 0.12
-
 ditrans,polycis-undecaprenyl phosphate pH 8, 65°C Thermotoga maritima
2.7.8.33 0.62
-
 UDP-N-acetyl-D-glucosamine pH 8, 65°C Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.33 membrane integral membrane protein Thermotoga maritima 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.33 Mg2+ absolute requirement for a divalent cation (Mg2+), optimal activity at 10 mM, inhibitory at higher conventrations, no activity in absence of Mg2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.33 30000
-
 SDS-PAGE Thermotoga maritima
2.7.8.33 33876
-
 x * 33876, calculated from sequence Thermotoga maritima
2.7.8.33 33880
-
 calculated from sequence Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate Thermotoga maritima the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.33 Thermotoga maritima Q9X1N5
-
-
2.7.8.33 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 Q9X1N5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.33 n-dodecyl-beta-D-maltoside is the most efficient detergent for both the extraction and purification steps of the WecA protein Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.33 0.2895
-
 pH 8, 65°C Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.33 additional information no activity with farnesyl phosphate and geranylgeranyl phosphate. Heptaprenyl phosphate shows 3% of the activity compared to ditrans,polycis-undecaprenyl phosphate Thermotoga maritima ?
-
 ?
2.7.8.33 additional information no activity with farnesyl phosphate and geranylgeranyl phosphate. Heptaprenyl phosphate shows 3% of the activity compared to ditrans,polycis-undecaprenyl phosphate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate a minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate a minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + dodecaprenyl phosphate about 85% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-dodecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + dodecaprenyl phosphate about 85% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-dodecaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + heptaprenyl phosphate about 80% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-heptaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + heptaprenyl phosphate about 80% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-heptaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + octaprenyl phosphate as active as ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-octaprenol
-
 ?
2.7.8.33 UDP-N-acetyl-D-glucosamine + pentadecaprenyl phosphate about 70% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-pentadecaprenol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.33 ? x * 30000, SDS-PAGE Thermotoga maritima
2.7.8.33 ? x * 33876, calculated from sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.8.33 UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase
-
 Thermotoga maritima
2.7.8.33 WecA
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.33 65
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.8.33 50 67 50°C: about 50% of maximal activity, 67°C: about 50% of maximal activity Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.33 1.2
-
 UDP-N-acetyl-D-glucosamine pH 8, 65°C Thermotoga maritima
2.7.8.33 1.2
-
 ditrans,polycis-undecaprenyl phosphate pH 8, 65°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.33 8
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.33 6.5 9.5 pH 6.5: about 50% of maximal activity, pH 9.5: about 70% of maximal activity Thermotoga maritima