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Literature summary extracted from

  • Cianci, M.; Tomaszewski, B.; Helliwell, J.R.; Halling, P.J.
    Crystallographic analysis of counterion effects on subtilisin enzymatic action in acetonitrile (2010), J. Am. Chem. Soc., 132, 2293-2300.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.62 choline increases the enzyme activity Bacillus licheniformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.62 by the batch method from buffer solution saturated with Na2SO4 ca. 13% (w/v) as precipitant. Placement of several Cs+ and Cl- ions in crystals of subtilisin Carlsberg. The protein conformation is very similar to that of the enzyme without CsCl in acetonitrile. 11 defined sites for Cs+ cations and 8 Cl- anions around the protein molecule, although most of these have partial occupancy and may represent nonspecific binding sites. Two Cs+ and two Cl- ions are close to the mouth of the active site cleft, where they may affect catalysis. CsCl-treated subtilisin crystals transferred to acetonitrile show catalytic activity several fold higher than the reference crystals containing Na+ Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.62 Cl- treatment with CsCl increases the activity several fold. Two Cl- ions are close to the mouth of the active site cleft, where they may affect catalysis Bacillus licheniformis
3.4.21.62 Cs+ treatment with CsCl increases the activity several fold. Two Cs+ ions are close to the mouth of the active site cleft, where they may affect catalysis Bacillus licheniformis
3.4.21.62 KCl increases enzymatic activity somewhat, while choline-Cl has a larger effect Bacillus licheniformis
3.4.21.62 additional information enzymatic activity of subtilisin crystals in acetonitrile is sensitive to the type of counterions present before transfer to the organic solvent. Larger cations increase the enzyme activity Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus licheniformis P00780
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 N-acetyl-Tyr ethyl ester + H2O
-
Bacillus licheniformis N-acetyl-Tyr + ethanol
-
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Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 subtilisin Carlsberg
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Bacillus licheniformis