Literature summary extracted from
Taira, T.; Hayashi, H.; Tajiri, Y.; Onaga, S.; Uechi, G.; Iwasaki, H.; Ohnuma, T.; Fukamizo, T.
A plant class V chitinase from a cycad (Cycas revoluta): biochemical characterization, cDNA isolation, and posttranslational modification (2009), Glycobiology, 19, 1452-1461.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.14 |
- |
Cycas revoluta |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.14 |
38000 |
- |
x * 38000, SDS-PAGE of mature protein |
Cycas revoluta |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.14 |
Cycas revoluta |
Q4W6L6 |
isoform ChiA |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.2.1.14 |
glycoprotein |
mature pürotein is N-glycosylated at residue N222 |
Cycas revoluta |
3.2.1.14 |
proteolytic modification |
sequence contains a N-terminal signal peptide, mature protein comprises residues Q24-G370 |
Cycas revoluta |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.14 |
- |
Cycas revoluta |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.2.1.14 |
leaf |
leaf rachise |
Cycas revoluta |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.14 |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O |
- |
Cycas revoluta |
2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc |
main product, minor products (GlcNAc)2 and (GlcNAc)4 are produced in the molar ratio (GlcNAc)3:(GlcNAc)2:(GlcNAc)4 of 1:0.3:0.4 |
? |
|
3.2.1.14 |
additional information |
enzyme exhibits transglycosylation activity |
Cycas revoluta |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.14 |
? |
x * 38000, SDS-PAGE of mature protein |
Cycas revoluta |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.14 |
50 |
60 |
- |
Cycas revoluta |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.14 |
4 |
5 |
- |
Cycas revoluta |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
3.2.1.14 |
Cycas revoluta |
isoelectric focusing |
- |
5.6 |