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Literature summary extracted from
- A plant class V chitinase from a cycad (Cycas revoluta): biochemical characterization, cDNA isolation, and posttranslational modification (2009), Glycobiology, 19, 1452-1461.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | - |
Cycas revoluta |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 38000 | - |
x * 38000, SDS-PAGE of mature protein | Cycas revoluta |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Cycas revoluta | Q4W6L6 | isoform ChiA | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | glycoprotein | mature pürotein is N-glycosylated at residue N222 | Cycas revoluta |
| 3.2.1.14 | proteolytic modification | sequence contains a N-terminal signal peptide, mature protein comprises residues Q24-G370 | Cycas revoluta |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | - |
Cycas revoluta |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | leaf | leaf rachise | Cycas revoluta | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Cycas revoluta | 2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | main product, minor products (GlcNAc)2 and (GlcNAc)4 are produced in the molar ratio (GlcNAc)3:(GlcNAc)2:(GlcNAc)4 of 1:0.3:0.4 | ? |  |
| 3.2.1.14 | additional information | enzyme exhibits transglycosylation activity | Cycas revoluta | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 38000, SDS-PAGE of mature protein | Cycas revoluta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 50 | 60 | - |
Cycas revoluta |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | 5 | - |
Cycas revoluta |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.14 | Cycas revoluta | isoelectric focusing | - |
5.6 |
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