Literature summary extracted from
Chatterjee, S.; Ehrenshaft, M.; Bhattacharjee, S.; Deterding, L.; Bonini, M.; Corbett, J.; Kadiiska, M.; Tomer, K.; Mason, R.
Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice (2009), Free Radic. Biol. Med., 46, 454-461.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.4.17.2 |
allopurinol |
addition of allopurinol alone and allopurinol plus 5,5-dimethyl-1-pyrroline N-oxide results in increased CPB1 activity |
Mus musculus |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.17.2 |
lipopolysaccharide |
lipopolysaccharide treatment significantly decreases carboxypeptidase B activity. Administration of 5,5-dimethyl-1-pyrroline N-oxide to LPS-treated mice alleviates this loss of enzyme activity |
Mus musculus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.17.2 |
Mus musculus |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.4.17.2 |
nitrosylation |
a post-translational DMPO-nitrone adduct formed with CPB1 in mice treated with a single bolus dose of lipopolysaccharide is detected |
Mus musculus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.4.17.2 |
spleen |
- |
Mus musculus |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.17.2 |
Carboxypeptidase B1 |
- |
Mus musculus |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.4.17.2 |
metabolism |
nitrone spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and a combination of immuno-spin trapping and mass spectrometry is used to identify in vivo products of radical reactions in mice. Dose-dependent production of 5,5-dimethyl-1-pyrroline N-oxide-CPB1 adducts are detected in the spleens of mice treated with lipopolysaccharide and also under normal physiological conditions. Treatments with inhibitors and experiments with knock-out mice indicate that xanthine oxidase and endothelial nitric oxide synthase are important sources of the reactive species that lead to CPB1 adduct formation |
Mus musculus |