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Literature summary extracted from

  • Guo, Y.L.; Mayer, H.; Vollmer, W.; Dittrich, D.; Sander, P.; Schultz, A.; Schultz, J.E.
    Polyphosphates from Mycobacterium bovis--potent inhibitors of class III adenylate cyclases (2009), FEBS J., 276, 1094-1103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expressed in Escherichia coli BL21(DE3)(pRep4) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.1 cyclic poly-phosphate 17
-
 Mycobacterium tuberculosis
4.6.1.1 cyclic triphosphate
-
 Mycobacterium tuberculosis
4.6.1.1 diphosphate
-
 Mycobacterium tuberculosis
4.6.1.1 additional information class I AC is unaffected by up to 0.03 mM poly-phosphate 75 Mycobacterium tuberculosis
4.6.1.1 phosphate
-
 Mycobacterium tuberculosis
4.6.1.1 poly-phosphate 16
-
 Mycobacterium tuberculosis
4.6.1.1 poly-phosphate 25
-
 Mycobacterium tuberculosis
4.6.1.1 poly-phosphate 30
-
 Mycobacterium tuberculosis
4.6.1.1 poly-phosphate 45
-
 Mycobacterium tuberculosis
4.6.1.1 poly-phosphate 68
-
 Mycobacterium tuberculosis
4.6.1.1 poly-phosphate 75
-
 Mycobacterium tuberculosis
4.6.1.1 Triphosphate
-
 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 membrane
-
 Mycobacterium tuberculosis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.1 ATP Mycobacterium tuberculosis
-
 3',5'-cyclic-AMP + diphosphate
-
 ?
4.6.1.1 ATP Mycobacterium tuberculosis BCG 1721
-
 3',5'-cyclic-AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Mycobacterium tuberculosis
-
-
-
4.6.1.1 Mycobacterium tuberculosis BCG 1721
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Mycobacterium tuberculosis 3',5'-cyclic-AMP + diphosphate
-
 ?
4.6.1.1 ATP
-
 Mycobacterium tuberculosis BCG 1721 3',5'-cyclic-AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 AC Rv1625c
-
 Mycobacterium tuberculosis
4.6.1.1 class III adenylate cyclase
-
 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.6.1.1 0.000011
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 75
4.6.1.1 0.000012
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 45
4.6.1.1 0.000012
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 68
4.6.1.1 0.000018
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 30
4.6.1.1 0.00002
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis Triphosphate
4.6.1.1 0.000027
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 25
4.6.1.1 0.000033
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis cyclic poly-phosphate 17
4.6.1.1 0.0001
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 16
4.6.1.1 0.00021
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis diphosphate
4.6.1.1 0.002
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis cyclic triphosphate
4.6.1.1 11
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis phosphate