Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kieper, N.; Holmstroem, K.M.; Ciceri, D.; Fiesel, F.C.; Wolburg, H.; Ziviani, E.; Whitworth, A.J.; Martins, L.M.; Kahle, P.J.; Krueger, R.
    Modulation of mitochondrial function and morphology by interaction of Omi/HtrA2 with the mitochondrial fusion factor OPA1 (2010), Exp. Cell Res., 316, 1213-1224.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 expressed in human embryonic kindney cells and in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 S306A mutant shows abolished protease activity Drosophila melanogaster
3.4.21.108 S306A mutant shows abolished protease activity Mus musculus
3.4.21.108 S306A mutant shows abolished protease activity Homo sapiens
3.4.21.108 S306A protease dead mutant Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.108 cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.108 35000
-
 endogenous Omi/HtrA2, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.108 additional information Homo sapiens co-immunoprecipitation show direct interaction of Omi/HtrA2 with optic atrophy protein 1 (OPA1) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Drosophila melanogaster
-
-
-
3.4.21.108 Homo sapiens
-
-
-
3.4.21.108 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.108 brain
-
 Mus musculus
-
3.4.21.108 MEF cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 beta-casein + H2O
-
 Mus musculus ?
-
 ?
3.4.21.108 additional information co-immunoprecipitation show direct interaction of Omi/HtrA2 with optic atrophy protein 1 (OPA1) Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 high temperature requirement protein A2
-
 Mus musculus
3.4.21.108 HtrA2
-
 Mus musculus
3.4.21.108 Omi
-
 Mus musculus
3.4.21.108 Omi/HtrA2
-
 Drosophila melanogaster
3.4.21.108 Omi/HtrA2
-
 Mus musculus
3.4.21.108 Omi/HtrA2
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.21.108 malfunction in Omi/HtrA2 knockout mouse embryonic fibroblasts elongated mitochondria are found by live cell imaging. Electron microscopy confirm the mitochondrial morphology alterations and show abnormal cristae structure. Examining the levels of proteins involved in mitochondrial fusion, a selective up-regulation of optic atrophy protein 1 is shown. Complementation of knockout cells with human wild-type Omi/HtrA2 reverses the mitochondrial elongation phenotype and optic atrophy protein 1 alterations Mus musculus
3.4.21.108 malfunction in Omi/HtrA2 silenced Drosophila S2R+ cells elongated mitochondria are found by live cell imaging. Electron microscopy confirm the mitochondrial morphology alterations and show abnormal cristae structure. Examining the levels of proteins involved in mitochondrial fusion, a selective up-regulation of optic atrophy protein 1 is shown Drosophila melanogaster
3.4.21.108 malfunction in Omi/HtrA2 silenced human HeLa elongated mitochondria are found by live cell imaging. Electron microscopy confirm the mitochondrial morphology alterations and show abnormal cristae structure. Examining the levels of proteins involved in mitochondrial fusion, a selective up-regulation of optic atrophy protein 1 is shown Homo sapiens
3.4.21.108 malfunction Omi/HtrA2 knockout mice show significant increase in intramitochondrial reactive oxygen species and decreased mitochondrial membrane potential in MEF cells Mus musculus
3.4.21.108 physiological function Omi/HtrA2 functionally and physically interacts with the mitochondrial fusion protein OPA1. Mitochondrial morphology relies on Omi/HtrA2 protease function Mus musculus