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Literature summary extracted from

  • Breitenbach, J.; Kuntz, M.; Takaichi, S.; Sandmann, G.
    Catalytic properties of an expressed and purified higher plant type zeta-carotene desaturase from Capsicum annuum (1999), Eur. J. Biochem., 265, 376-383.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.5.6 decyl-plastoquinone enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate Capsicum annuum
1.3.5.6 decyl-ubiquinone enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate Capsicum annuum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.6 expression in Escherichia coli Capsicum annuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.6 0.0084
-
 zeta-carotene pH 7.8, 28°C Capsicum annuum
1.3.5.6 0.009
-
 Neurosporene pH 7.8, 28°C Capsicum annuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.6 60000
-
 x * 60000, SDS-PAGE Capsicum annuum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.6 neurosporene + a quinone Capsicum annuum
-
 lycopene + a quinol
-
 ?
1.3.5.6 zeta-carotene + 2 quinone Capsicum annuum zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers lycopene + 2 quinol
-
 ?
1.3.5.6 zeta-carotene + a quinone Capsicum annuum
-
 neurosporene + a quinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.6 Capsicum annuum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.6
-
 Capsicum annuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.6 neurosporene + a quinone
-
 Capsicum annuum lycopene + a quinol
-
 ?
1.3.5.6 neurosporene + decyl-plastoquinone
-
 Capsicum annuum lycopene + decyl-plastoquinol
-
 ?
1.3.5.6 neurosporene + decyl-ubiquinone
-
 Capsicum annuum lycopene + decyl-ubiquinone
-
 ?
1.3.5.6 zeta-carotene + 2 decyl-plastoquinone zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers Capsicum annuum lycopene + 2 decyl-plastoquinol
-
 ?
1.3.5.6 zeta-carotene + 2 decyl-ubiquinone zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers Capsicum annuum lycopene + 2 decyl-ubiquinone
-
 ?
1.3.5.6 zeta-carotene + 2 quinone zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers Capsicum annuum lycopene + 2 quinol
-
 ?
1.3.5.6 zeta-carotene + a quinone
-
 Capsicum annuum neurosporene + a quinol
-
 ?
1.3.5.6 zeta-carotene + decyl-plastoquinone
-
 Capsicum annuum neurosporene + decyl-plastoquinol
-
 ?
1.3.5.6 zeta-carotene + decyl-ubiquinone
-
 Capsicum annuum neurosporene + decyl-ubiquinone
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.6 ? x * 60000, SDS-PAGE Capsicum annuum

Synonyms

EC Number Synonyms Comment Organism
1.3.5.6 zeta-carotene desaturase
-
-
1.3.5.6 zeta-carotene desaturase
-
 Capsicum annuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.5.6 28
-
 assay at Capsicum annuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.6 7.8
-
 assay at Capsicum annuum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.6 additional information addition of the dinucleotides FAD, NADP+ or NAD+ alone or the latter in combination with ATP results in a negligible increase in zeta-carotene desaturase activity Capsicum annuum
1.3.5.6 quinone
-
 Capsicum annuum