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Literature summary extracted from
- Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica (1993), Eur. J. Biochem., 212, 819-826.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.36 | additional information | NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity | Amycolatopsis methanolica | |
| 1.1.99.36 | additional information | no activation by phosphate and ammonium chloride. NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity | Amycolatopsis methanolica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.36 | 1-butanol | substrate inhibition | Amycolatopsis methanolica |  |
| 1.1.99.36 | 1-propanol | substrate inhibition | Amycolatopsis methanolica | |
| 1.1.99.36 | 2-butanol | substrate inhibition | Amycolatopsis methanolica | |
| 1.1.99.36 | 2-propanol | substrate inhibition | Amycolatopsis methanolica | |
| 1.1.99.36 | acetaldehyde | 1 mM, 40% inhibition; 40% inhibition at 1 mM, 60% at 2 mM, and 95% at 10 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | ADP | 10% inhibition at 1 mM, 50% at 10 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | AMP | 10% inhibition at 1 mM, 50% at 10 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | ATP | 10% inhibition at 1 mM, 50% at 10 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | Cu2+ | 1 mM, 90% inhibition; 90% inhibition at 1 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | ethanol | substrate inhibition | Amycolatopsis methanolica | |
| 1.1.99.36 | Fe2+ | 1 mM, 90% inhibition; 90% inhibition at 1 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | Hg2+ | 100% inhibition at 1 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | KCN | 2 mM, 90% inhibition; 90% inhibition at 2 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | additional information | NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity; NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity | Amycolatopsis methanolica | |
| 1.1.99.36 | NaN3 | 25% inhibition at 2 mM; 2 mM, 25% inhibition | Amycolatopsis methanolica | |
| 1.1.99.36 | Ni2+ | 15% inhibition at 1 mM | Amycolatopsis methanolica | |
| 1.1.99.36 | Zn2+ | 1 mM, 40% inhibition; 40% inhibition at 1 mM | Amycolatopsis methanolica | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.36 | additional information | - |
additional information | kinetics | Amycolatopsis methanolica | |
| 1.1.99.36 | 0.0025 | - |
1-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 0.0039 | - |
1-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 0.082 | - |
ethanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 10.1 | - |
2-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 13.4 | - |
2-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.36 | 39000 | - |
3 * 39000, SDS-PAGE | Amycolatopsis methanolica |
| 1.1.99.36 | 110000 | - |
PAGE | Amycolatopsis methanolica |
| 1.1.99.36 | 110000 | - |
native PAGE | Amycolatopsis methanolica |
| 1.1.99.36 | 120000 | - |
gel filtration | Amycolatopsis methanolica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.36 | ethanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica | - |
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | ethanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica NCIB 11946 | - |
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | methanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica | - |
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | methanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica NCIB 11946 | - |
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.36 | Amycolatopsis methanolica | - |
- |
- |
| 1.1.99.36 | Amycolatopsis methanolica | P80175 | - |
- |
| 1.1.99.36 | Amycolatopsis methanolica NCIB 11946 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.36 | native enzyme 163fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, anion exchange and hydroxyapatite chromatography, followed by gel filtration | Amycolatopsis methanolica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.36 | 0.003 | - |
substrate methanol, pH 7.0, 45°C, extracts of cells grown on ethanol | Amycolatopsis methanolica |
| 1.1.99.36 | 0.004 | - |
substrate ethanol, pH 7.0, 45°C, extracts of cells grown on ethanol | Amycolatopsis methanolica |
| 1.1.99.36 | 0.005 | - |
substrate methanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol | Amycolatopsis methanolica |
| 1.1.99.36 | 0.018 | - |
substrate ethanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol | Amycolatopsis methanolica |
| 1.1.99.36 | 3.1 | - |
45°C, pH 7.0, purified enzyme | Amycolatopsis methanolica |
| 1.1.99.36 | 3.1 | - |
purified enzyme, pH 7.0, 45°C | Amycolatopsis methanolica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.36 | 1-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | butanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | 1-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | butanal + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | 1-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica NCIB 11946 | butanal + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | 1-hexanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | hexanal + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | 1-propanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | propanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | 2-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | butanone + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | 2-propanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | acetone + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | acetate + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | ? + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | ethanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | ethanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica NCIB 11946 | acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | methanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | methanol + N,N-dimethyl-4-nitrosoaniline | best substrate | Amycolatopsis methanolica | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | methanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica NCIB 11946 | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | methanol + N,N-dimethyl-4-nitrosoaniline | best substrate | Amycolatopsis methanolica NCIB 11946 | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
| 1.1.99.36 | additional information | enzyme shows formaldehyde dismutase activity producing methylformate when incubated with formaldehyde and methanol | Amycolatopsis methanolica | ? | - |
? | |
| 1.1.99.36 | additional information | no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c | Amycolatopsis methanolica | ? | - |
? | |
| 1.1.99.36 | additional information | no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c | Amycolatopsis methanolica NCIB 11946 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.36 | trimer | 3 * 39000, SDS-PAGE | Amycolatopsis methanolica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.36 | 4-nitroso-N, N-dimethylaniline-dependent alcohol dehydrogenase | - |
Amycolatopsis methanolica |
| 1.1.99.36 | NDMA-ADH | - |
Amycolatopsis methanolica |
| 1.1.99.36 | NDMA-dependent alcohol dehydrogenase | - |
Amycolatopsis methanolica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.36 | 45 | - |
- |
Amycolatopsis methanolica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.36 | 7 | - |
- |
Amycolatopsis methanolica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.36 | additional information | no cofactor: NADPH | Amycolatopsis methanolica | |
| 1.1.99.36 | NADH | 1 mol NADH per mol of enzyme trimer | Amycolatopsis methanolica | |
| 1.1.99.36 | NADH | tightly bound, but NDMA-dependent alcohol dehydrogenase is unable to use NAD(P)(H) as cofactors | Amycolatopsis methanolica | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.36 | 0.65 | - |
1-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 4 | - |
1-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 4.4 | - |
2-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 14.5 | - |
2-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
| 1.1.99.36 | 33 | - |
ethanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
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