BRENDA - Enzyme Database show

Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica

Van Ophem, P.W.; Van Beeumen, J.; Duine, J.A.; Eur. J. Biochem. 212, 819-826 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.99.36
additional information
NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity; no activation by phosphate and ammonium chloride. NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity
Amycolatopsis methanolica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.36
1-butanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
1-propanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
2-butanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
2-propanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
acetaldehyde
1 mM, 40% inhibition; 40% inhibition at 1 mM, 60% at 2 mM, and 95% at 10 mM
Amycolatopsis methanolica
1.1.99.36
ADP
10% inhibition at 1 mM, 50% at 10 mM
Amycolatopsis methanolica
1.1.99.36
AMP
10% inhibition at 1 mM, 50% at 10 mM
Amycolatopsis methanolica
1.1.99.36
ATP
10% inhibition at 1 mM, 50% at 10 mM
Amycolatopsis methanolica
1.1.99.36
Cu2+
1 mM, 90% inhibition; 90% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
ethanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
Fe2+
1 mM, 90% inhibition; 90% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
Hg2+
100% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
KCN
2 mM, 90% inhibition; 90% inhibition at 2 mM
Amycolatopsis methanolica
1.1.99.36
additional information
NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity; NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity
Amycolatopsis methanolica
1.1.99.36
NaN3
25% inhibition at 2 mM; 2 mM, 25% inhibition
Amycolatopsis methanolica
1.1.99.36
Ni2+
15% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
Zn2+
1 mM, 40% inhibition; 40% inhibition at 1 mM
Amycolatopsis methanolica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.99.36
additional information
-
additional information
kinetics
Amycolatopsis methanolica
1.1.99.36
0.0025
-
1-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
0.0039
-
1-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
0.082
-
ethanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
10.1
-
2-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
13.4
-
2-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.36
39000
-
3 * 39000, SDS-PAGE; 3 * 39000, SDS-PAGE
Amycolatopsis methanolica
1.1.99.36
110000
-
native PAGE; PAGE
Amycolatopsis methanolica
1.1.99.36
120000
-
gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica
-
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica NCIB 11946
-
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica
-
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica NCIB 11946
-
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.99.36
Amycolatopsis methanolica
-
-
-
1.1.99.36
Amycolatopsis methanolica
P80175
-
-
1.1.99.36
Amycolatopsis methanolica NCIB 11946
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.99.36
native enzyme 163fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, anion exchange and hydroxyapatite chromatography, followed by gel filtration
Amycolatopsis methanolica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.99.36
0.003
-
substrate methanol, pH 7.0, 45°C, extracts of cells grown on ethanol
Amycolatopsis methanolica
1.1.99.36
0.004
-
substrate ethanol, pH 7.0, 45°C, extracts of cells grown on ethanol
Amycolatopsis methanolica
1.1.99.36
0.005
-
substrate methanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol
Amycolatopsis methanolica
1.1.99.36
0.018
-
substrate ethanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol
Amycolatopsis methanolica
1.1.99.36
3.1
-
45°C, pH 7.0, purified enzyme; purified enzyme, pH 7.0, 45°C
Amycolatopsis methanolica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.36
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
butanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
butanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica NCIB 11946
butanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-hexanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
hexanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-propanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
propanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
2-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
butanone + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
2-propanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
acetone + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
acetate + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
? + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica NCIB 11946
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
best substrate
708364
Amycolatopsis methanolica
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica NCIB 11946
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
best substrate
708364
Amycolatopsis methanolica NCIB 11946
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
additional information
enzyme shows formaldehyde dismutase activity producing methylformate when incubated with formaldehyde and methanol
708364
Amycolatopsis methanolica
?
-
-
-
-
1.1.99.36
additional information
no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c
708364
Amycolatopsis methanolica
?
-
-
-
-
1.1.99.36
additional information
no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c
708364
Amycolatopsis methanolica NCIB 11946
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.99.36
trimer
3 * 39000, SDS-PAGE; 3 * 39000, SDS-PAGE
Amycolatopsis methanolica
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.99.36
45
-
;
Amycolatopsis methanolica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.99.36
7
-
;
Amycolatopsis methanolica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.36
additional information
no cofactor: NADPH
Amycolatopsis methanolica
1.1.99.36
NADH
1 mol NADH per mol of enzyme trimer; tightly bound, but NDMA-dependent alcohol dehydrogenase is unable to use NAD(P)(H) as cofactors
Amycolatopsis methanolica
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.99.36
0.65
-
1-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
4
-
1-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
4.4
-
2-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
14.5
-
2-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
33
-
ethanol
pH 7.0, 45°C
Amycolatopsis methanolica
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.99.36
additional information
NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity
Amycolatopsis methanolica
1.1.99.36
additional information
no activation by phosphate and ammonium chloride. NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity
Amycolatopsis methanolica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.36
additional information
no cofactor: NADPH
Amycolatopsis methanolica
1.1.99.36
NADH
1 mol NADH per mol of enzyme trimer
Amycolatopsis methanolica
1.1.99.36
NADH
tightly bound, but NDMA-dependent alcohol dehydrogenase is unable to use NAD(P)(H) as cofactors
Amycolatopsis methanolica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.36
1-butanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
1-propanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
2-butanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
2-propanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
acetaldehyde
1 mM, 40% inhibition
Amycolatopsis methanolica
1.1.99.36
acetaldehyde
40% inhibition at 1 mM, 60% at 2 mM, and 95% at 10 mM
Amycolatopsis methanolica
1.1.99.36
ADP
10% inhibition at 1 mM, 50% at 10 mM
Amycolatopsis methanolica
1.1.99.36
AMP
10% inhibition at 1 mM, 50% at 10 mM
Amycolatopsis methanolica
1.1.99.36
ATP
10% inhibition at 1 mM, 50% at 10 mM
Amycolatopsis methanolica
1.1.99.36
Cu2+
1 mM, 90% inhibition
Amycolatopsis methanolica
1.1.99.36
Cu2+
90% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
ethanol
substrate inhibition
Amycolatopsis methanolica
1.1.99.36
Fe2+
1 mM, 90% inhibition
Amycolatopsis methanolica
1.1.99.36
Fe2+
90% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
Hg2+
100% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
KCN
2 mM, 90% inhibition
Amycolatopsis methanolica
1.1.99.36
KCN
90% inhibition at 2 mM
Amycolatopsis methanolica
1.1.99.36
additional information
NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity
Amycolatopsis methanolica
1.1.99.36
additional information
NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity
Amycolatopsis methanolica
1.1.99.36
NaN3
2 mM, 25% inhibition
Amycolatopsis methanolica
1.1.99.36
NaN3
25% inhibition at 2 mM
Amycolatopsis methanolica
1.1.99.36
Ni2+
15% inhibition at 1 mM
Amycolatopsis methanolica
1.1.99.36
Zn2+
1 mM, 40% inhibition
Amycolatopsis methanolica
1.1.99.36
Zn2+
40% inhibition at 1 mM
Amycolatopsis methanolica
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.99.36
0.65
-
1-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
4
-
1-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
4.4
-
2-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
14.5
-
2-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
33
-
ethanol
pH 7.0, 45°C
Amycolatopsis methanolica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.99.36
additional information
-
additional information
kinetics
Amycolatopsis methanolica
1.1.99.36
0.0025
-
1-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
0.0039
-
1-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
0.082
-
ethanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
10.1
-
2-butanol
pH 7.0, 45°C
Amycolatopsis methanolica
1.1.99.36
13.4
-
2-propanol
pH 7.0, 45°C
Amycolatopsis methanolica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.36
39000
-
3 * 39000, SDS-PAGE
Amycolatopsis methanolica
1.1.99.36
110000
-
PAGE
Amycolatopsis methanolica
1.1.99.36
110000
-
native PAGE
Amycolatopsis methanolica
1.1.99.36
120000
-
gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica
-
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica NCIB 11946
-
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica
-
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
Amycolatopsis methanolica NCIB 11946
-
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.99.36
native enzyme 163fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, anion exchange and hydroxyapatite chromatography, followed by gel filtration
Amycolatopsis methanolica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.99.36
0.003
-
substrate methanol, pH 7.0, 45°C, extracts of cells grown on ethanol
Amycolatopsis methanolica
1.1.99.36
0.004
-
substrate ethanol, pH 7.0, 45°C, extracts of cells grown on ethanol
Amycolatopsis methanolica
1.1.99.36
0.005
-
substrate methanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol
Amycolatopsis methanolica
1.1.99.36
0.018
-
substrate ethanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol
Amycolatopsis methanolica
1.1.99.36
3.1
-
45°C, pH 7.0, purified enzyme
Amycolatopsis methanolica
1.1.99.36
3.1
-
purified enzyme, pH 7.0, 45°C
Amycolatopsis methanolica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.36
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
butanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
butanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica NCIB 11946
butanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-hexanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
hexanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
1-propanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
propanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
2-butanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
butanone + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
2-propanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
acetone + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
acetate + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
? + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
ethanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica NCIB 11946
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
best substrate
708364
Amycolatopsis methanolica
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
-
708364
Amycolatopsis methanolica NCIB 11946
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
methanol + N,N-dimethyl-4-nitrosoaniline
best substrate
708364
Amycolatopsis methanolica NCIB 11946
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
-
-
?
1.1.99.36
additional information
enzyme shows formaldehyde dismutase activity producing methylformate when incubated with formaldehyde and methanol
708364
Amycolatopsis methanolica
?
-
-
-
-
1.1.99.36
additional information
no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c
708364
Amycolatopsis methanolica
?
-
-
-
-
1.1.99.36
additional information
no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c
708364
Amycolatopsis methanolica NCIB 11946
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.99.36
trimer
3 * 39000, SDS-PAGE
Amycolatopsis methanolica
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.99.36
45
-
-
Amycolatopsis methanolica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.99.36
7
-
-
Amycolatopsis methanolica