Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fu, D.; Huang, H.; Luo, H.; Wang, Y.; Yang, P.; Meng, K.; Bai, Y.; Wu, N.; Yao, B.
    A highly pH-stable phytase from Yersinia kristeensenii: Cloning, expression, and characterization (2008), Enzyme Microb. Technol., 42, 499-505.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Pichia pastoris Yersinia kristensenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Cu2+ 61% residual activity Yersinia kristensenii
3.1.3.8 Fe3+ complete inhibition Yersinia kristensenii
3.1.3.8 Hg2+ 10% residual activity Yersinia kristensenii
3.1.3.8 Pd2+ 25% residual activity Yersinia kristensenii
3.1.3.8 SDS complete inhibition Yersinia kristensenii
3.1.3.8 Zn2+ 35% residual activity Yersinia kristensenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.078
-
 myo-inositol hexakisphosphate pH 4.5, 55°C Yersinia kristensenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 45900
-
 x * 45900, calculated for mature protein, x * 48000, SDS-PAGE Yersinia kristensenii
3.1.3.8 48000
-
 x * 45900, calculated for mature protein, x * 48000, SDS-PAGE Yersinia kristensenii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Yersinia kristensenii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein sequence contains a putative N-glycosylation site at residue R198, molecular mass decreases after treatment with Endo H Yersinia kristensenii
3.1.3.8 proteolytic modification sequence contains a signal peptide of 24 amino acids Yersinia kristensenii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 2656
-
 pH 4.5, 55°C Yersinia kristensenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Yersinia kristensenii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 soybean meal + H2O
-
 Yersinia kristensenii ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 45900, calculated for mature protein, x * 48000, SDS-PAGE Yersinia kristensenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
-
 Yersinia kristensenii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 80
-
 10 min, 46% residual activity Yersinia kristensenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4.5
-
-
 Yersinia kristensenii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2 6.5
-
 Yersinia kristensenii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 1.5 11 37°C, 3 h, more than 90% of initial activity Yersinia kristensenii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Yersinia kristensenii calculated
-
 6.2