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Literature summary extracted from

  • Haga, Y.; Miwa, N.; Jahangeer, S.; Okada, T.; Nakamura, S.
    CtBP1/BARS is an activator of phospholipase D1 necessary for agonist-induced macropinocytosis (2009), EMBO J., 28, 1197-1207.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 Arf1 protein activates PLD, acts synergistically with CtBP1/BARS in activation of PLD, dependent on GTPgammaS or GTP but not on GDP, overview Rattus norvegicus
3.1.4.4 CtBP1/BARS a short splice variant of the CtBP1 gene and a physiological activator of PLD1 required in agonist-induced macropinocytosis. Stimulation of cells by serum or EGF results in the association of CtBP1/BARS with PLD1, which is specifically blocked by 1-butanol. CtBP1/BARS acts synergistically with ARF1 protein in activation of PLD, dependent on GTPgammaS or GTP but not on GDP, overview Rattus norvegicus
3.1.4.4 additional information activation mechanism of PLD in macropinocytosis, overview Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 co-expression of the respective wild-type PLD isoforms or lipase-negative (LN)-PLD1 or LN-PLD2inactive mutants in PLD1 or PLD2 KO mutant cells, respectively Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 additional information the inhibition of EGF-induced macropinocytosis caused either by PLD1 or PLD2 knockdown is rescued by the co-expression of the respective wild-type PLD isoforms, whereas the expression of a lipase inactive mutant, lipase-negative (LN)-PLD1 or LN-PLD2, does not restore macropinocytotic activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 1-butanol
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 additional information Rattus norvegicus PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, 1-butanol serves as acceptor in the transphosphatidylation reaction, while 2-butanol does not. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Rattus norvegicus
-
 isozymes PLD1 and PLD2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, 1-butanol serves as acceptor in the transphosphatidylation reaction, while 2-butanol does not. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D1
-
 Rattus norvegicus
3.1.4.4 PLD1
-
 Rattus norvegicus
3.1.4.4 PLD2
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
3.1.4.4 physiological function vesicular trafficking such as macropinocytosis is a dynamic process that requires coordinated interactions between specialized proteins and lipids that involves PLD and its physiological activator, the fission protein CtBP1/BARS in agonist-induced macropinocytosis, overview. Molecular mechanisms of lipid remodelling regulation during macropinocytosis. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis Rattus norvegicus