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Literature summary extracted from

  • Fonseca, F.V.; Silva, J.R.; Samuels, R.I.; DaMatta, R.A.; Terra, W.R.; Silva, C.P.
    Purification and partial characterization of a midgut membrane-bound alpha-glucosidase from Quesada gigas (Hemiptera: Cicadidae) (2010), Comp. Biochem. Physiol. B, 155, 20-25.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.02
-
 maltotetraose
-
 Quesada gigas
3.2.1.20 1.34
-
 maltotriose
-
 Quesada gigas
3.2.1.20 1.58
-
 p-nitrophenyl alpha-D-glucoside
-
 Quesada gigas
3.2.1.20 3.27
-
 maltoheptaose
-
 Quesada gigas
3.2.1.20 3.62
-
 maltohexaose
-
 Quesada gigas
3.2.1.20 3.99
-
 maltopentaose
-
 Quesada gigas
3.2.1.20 5.46
-
 maltose
-
 Quesada gigas
3.2.1.20 10.8
-
 sucrose
-
 Quesada gigas

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 membrane perimicrovillar membranes Quesada gigas 16020
-
3.2.1.20 soluble
-
 Quesada gigas
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 61000
-
 SDS-PAGE Quesada gigas

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Quesada gigas
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 gel filtration and ion-exchange Quesada gigas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 midgut
-
 Quesada gigas
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucoside + H2O assay at pH 6.0, 30°C Quesada gigas 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 maltoheptaose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + ?
-
 ?
3.2.1.20 maltohexaose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + ?
-
 ?
3.2.1.20 maltopentaose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + ?
-
 ?
3.2.1.20 maltotetraose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + ?
-
 ?
3.2.1.20 maltotriose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + ?
-
 ?
3.2.1.20 sucrose + H2O assay at pH 6.0, 30°C Quesada gigas D-glucose + D-fructose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 30
-
 assay at Quesada gigas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6
-
-
 Quesada gigas

General Information

EC Number General Information Comment Organism
3.2.1.20 physiological function possible role of enzyme in xylem fluid digestion and in osmoregulation Quesada gigas