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Literature summary extracted from

  • Powlowski, J.; Mahajan, S.; Schapira, M.; Master, E.R.
    Substrate recognition and hydrolysis by a fungal xyloglucan-specific family 12 hydrolase (2009), Carbohydr. Res., 344, 1175-1179.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 0.54
-
 xyloglucan pH 5.0, 50°C, substrate from pea seed Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 xyloglucan + H2O Aspergillus niger
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Aspergillus niger
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.151 113
-
 substrate pea seed xyloglucan Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 additional information modelling of substrate binding, overview. Substrate fucosylation does not affect the specific activity of this enzyme. AnXEG12A prefers xylogluco-oligosaccharides containing more than six glucose units, and with xylose substitution at the -3 and +1 subsites, branching structures do not significantly affect the hydrolytic efficiency of the enzyme, overview Aspergillus niger ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus niger xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O pea seed xyloglucan Aspergillus niger xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan oligosaccharide + H2O diverse substrates, substrate specificity, overview Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 More AnXEG12A structure homology modelling, overview. Catalytic residues are D100, E115, and E201, predicted substrate binding residues are W22, and W119, and residues that are predicted to influence substrate specificity in GH12 are xyloglucanases W7, V94, and G127. The SST insertion sequence is loacted at residues 130-132 Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 GH12 xyloglucanase
-
 Aspergillus niger
3.2.1.151 XEG12A
-
 Aspergillus niger
3.2.1.151 xyloglucan-specific family 12 hydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 50
-
 assay at Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 49
-
 xyloglucan pH 5.0, 50°C, substrate from pea seed Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5
-
 assay at Aspergillus niger