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Literature summary extracted from

  • Fu, D.; Huang, H.; Meng, K.; Wang, Y.; Luo, H.; Yang, P.; Yuan, T.; Yao, B.
    Improvement of Yersinia frederiksenii phytase performance by a single amino acid substitution (2009), Biotechnol. Bioeng., 103, 857-864.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8
-
 Yersinia frederiksenii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 S51A optimum temperature and pH similar to wild-type Yersinia frederiksenii
3.1.3.8 S51D optimum temperature and pH similar to wild-type Yersinia frederiksenii
3.1.3.8 S51I shift in optimal pH to 5.0. Compared with wild-type, mutant S51T shows higher specific activity, greater activity over pH 2.0-5.5, and increased thermal and acid stability Yersinia frederiksenii
3.1.3.8 S51K optimum temperature and pH similar to wild-type Yersinia frederiksenii
3.1.3.8 S51T shift in optimal pH to 4.5. Specific activity of S51T at pH 4.5 is 5.5fold higher than the corresponding specific activity of wild-type Yersinia frederiksenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 45900
-
 x * 46000, SDS-PAGE, x * 45900, calculated Yersinia frederiksenii
3.1.3.8 46000
-
 x * 46000, SDS-PAGE, x * 45900, calculated Yersinia frederiksenii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Yersinia frederiksenii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 428
-
 pH 2.5, 37°C Yersinia frederiksenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O sodium salt Yersinia frederiksenii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 46000, SDS-PAGE, x * 45900, calculated Yersinia frederiksenii

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 AppA
-
 Yersinia frederiksenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 45
-
-
 Yersinia frederiksenii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
 2 min, pH 2.5, complete loss of activity for wild-type. Mutants S51I and S51T retain about 80% of activity Yersinia frederiksenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2.5
-
-
 Yersinia frederiksenii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 1 2 1 h, 37°C, unstable Yersinia frederiksenii
3.1.3.8 2 3 1 h, 37°C, loss of 60% of activity Yersinia frederiksenii