Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gupta, S.; Mahmood, S.; Khan, R.H.; Mahmood, A.
    Inhibition of brush border sucrase by polyphenols in mouse intestine (2010), Biosci. Rep., 30, 111-117.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.48 gallic acid inhibition of brush border sucrase by polyphenols in mouse intestine. Inhibition by gallic acid is a pure V effect at pH 5.0, which changes to mixed type at pH 7.2, and pure K effect at pH 8.5 Mus musculus
3.2.1.48 additional information inhibition of mice sucrase by polyphenols is pH-dependent, and is associated with conformational modifications of the enzyme. At pH 4.8, the polyphenols inhibit sucrase activity by 85-96%, which is reduced to 51 and 64%, respectively, at pH 7.2. However, at pH 8.5, 60 and 76% inhibition of enzyme activity Mus musculus
3.2.1.48 Tannic acid inhibition of brush border sucrase by polyphenols in mouse intestine. Sucrase inhibition by tannic acid is a pure K effect at acidic pH and uncompetitive type in the alkaline pH range Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.48 additional information
-
 additional information Km values in presence of inhibitors, at pH 7.2, both gallic acid and tannic acid show mixed-type enzyme inhibition, Km is enhanced by 77-114% and Vmax by 55-60%, overview Mus musculus
3.2.1.48 96.7
-
 sucrose pH 8.5 Mus musculus
3.2.1.48 130
-
 sucrose pH 5.0 Mus musculus
3.2.1.48 138
-
 sucrose pH 7.2 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.48 brush border
-
 Mus musculus 5903
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.48 210000
-
 x * 210000, about Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 sucrose + H2O Mus musculus
-
 alpha-D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.48 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.48 native enzyme from brush border 155fold by isolation from the microvillus membranes by papain, gel filtration and anion exchange chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.48 intestine
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.48 sucrose + H2O
-
 Mus musculus alpha-D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.48 ? x * 210000, about Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.48 7.2
-
 assay at Mus musculus