EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | EDTA | slight stimulation | Aspergillus ficuum | |
3.1.3.8 | EDTA | slight stimulation | Aspergillus ficuum | |
3.1.3.8 | EDTA | slight activation at 1-10 mM | Aspergillus ficuum |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.3.8 | agriculture | the phytase has the potential to be useful as an animal feed supplement | Aspergillus ficuum |
3.1.3.8 | nutrition | the phytase has the potential to be useful as an animal feed supplement | Aspergillus ficuum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.2 | - |
Aspergillus ficuum |
3.1.3.8 | - |
Aspergillus ficuum |
EC Number | General Stability | Organism |
---|---|---|
3.1.3.2 | enzyme shows strong resistance toward pepsin and trypsin | Aspergillus ficuum |
3.1.3.8 | enzyme shows strong resistance toward pepsin and trypsin | Aspergillus ficuum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | additional information | no effect: Ca2+, Mg2+, Mn2+, Zn2+ | Aspergillus ficuum | |
3.1.3.8 | additional information | no effect: Ca2+, Mg2+, Mn2+, Zn2+ | Aspergillus ficuum | |
3.1.3.8 | additional information | no activation or inhibition by cations K+, Ca2+, Mg2+, Mn2+, Zn2+, Cd2+, Cu2+, Pb2+, Hg2+, Fe3+, and Al3+ | Aspergillus ficuum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 65500 | - |
gel filtration | Aspergillus ficuum |
3.1.3.8 | 65500 | - |
gel filtration | Aspergillus ficuum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | phytate + H2O | Aspergillus ficuum | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Aspergillus ficuum | - |
- |
- |
3.1.3.2 | Aspergillus ficuum NTG-23 | - |
- |
- |
3.1.3.8 | Aspergillus ficuum | - |
- |
- |
3.1.3.8 | Aspergillus ficuum NTG-23 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | native enzyme 71.5fold by anion exchange chromatography, cation exchange chromatography, and gel filtration | Aspergillus ficuum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.8 | mycelium | - |
Aspergillus ficuum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 150 | - |
37°C, pH 2.5 | Aspergillus ficuum |
3.1.3.8 | 150 | - |
37°C, pH 2.5 | Aspergillus ficuum |
3.1.3.8 | 150.1 | - |
purified native enzyme, pH 2.5, 37°C | Aspergillus ficuum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | 2-glycerophosphate + H2O | 127% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | glycerate + phosphate | - |
? | |
3.1.3.2 | 4-nitrophenyl phosphate + H2O | 254% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
3.1.3.2 | ADP + H2O | 177% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | ? + phosphate | - |
? | |
3.1.3.2 | AMP + H2O | 173% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | adenosine + phosphate | - |
? | |
3.1.3.2 | ATP + H2O | 256% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | ? + phosphate | - |
? | |
3.1.3.2 | D-fructose 6-phosphate + H2O | 228% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | D-fructose + phosphate | - |
? | |
3.1.3.2 | D-glucose 6-phosphate + H2O | 190% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | D-glucose + phosphate | - |
? | |
3.1.3.2 | myo-inositol hexakisphosphate + H2O | sodium salt | Aspergillus ficuum | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
3.1.3.8 | 2-glycerophosphate + H2O | 127% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | glycerate + phosphate | - |
? | |
3.1.3.8 | 4-nitrophenyl phosphate + H2O | 254% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
3.1.3.8 | 4-nitrophenyl phosphate + H2O | 254% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | 4-nitrophenol + phosphate | - |
? | |
3.1.3.8 | ADP + H2O | 177% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | ? + phosphate | - |
? | |
3.1.3.8 | AMP + H2O | 173% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | adenosine + phosphate | - |
? | |
3.1.3.8 | AMP + H2O | 173% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | adenosine + phosphate | - |
? | |
3.1.3.8 | ATP + H2O | 256% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | ? + phosphate | - |
? | |
3.1.3.8 | D-fructose 6-phosphate + H2O | 228% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | D-fructose + phosphate | - |
? | |
3.1.3.8 | D-fructose 6-phosphate + H2O | 228% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | D-fructose + phosphate | - |
? | |
3.1.3.8 | D-glucose 6-phosphate + H2O | 190% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | D-glucose + phosphate | - |
? | |
3.1.3.8 | D-glucose 6-phosphate + H2O | 190% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | D-glucose + phosphate | - |
? | |
3.1.3.8 | additional information | the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate | Aspergillus ficuum | ? | - |
? | |
3.1.3.8 | additional information | the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate | Aspergillus ficuum NTG-23 | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | sodium salt | Aspergillus ficuum | myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
3.1.3.8 | phytate + H2O | - |
Aspergillus ficuum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.2 | monomer | 1 * 65500, SDS-PAGE | Aspergillus ficuum |
3.1.3.8 | monomer | 1 * 65500, SDS-PAGE | Aspergillus ficuum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 67 | - |
- |
Aspergillus ficuum |
3.1.3.8 | 67 | - |
- |
Aspergillus ficuum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 70 | - |
20 min, no loss of activity | Aspergillus ficuum |
3.1.3.8 | 60 | 70 | purified native enzyme, stable at 60°C, no obvious loss of activity upon incubation at 70°C for 20 min | Aspergillus ficuum |
3.1.3.8 | 70 | - |
20 min, no loss of activity | Aspergillus ficuum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 1.3 | - |
- |
Aspergillus ficuum |
3.1.3.8 | 1.3 | - |
- |
Aspergillus ficuum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.8 | additional information | the enzyme shows strong resistance toward pepsin and trypsin | Aspergillus ficuum |