Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cozzani, I.; Fazio, A.M.; Felici, E.; Barletta, G.
    Separation and characterization of NAD- and NADP-specific succinate-semialdehyde dehydrogenase from Escherichia coli K-12 3300 (1980), Biochim. Biophys. Acta, 613, 309-317.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.24 2-mercaptoethanol loss of about 50% of original acitivity after exhaustive dialysis, renaturation in presence of 2-mercaptoethanol Escherichia coli K-12
1.2.1.79 2-mercaptoethanol loss of about 80% of original acitivity after exhaustive dialysis, renaturation in presence of 2-mercaptoethanol Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.24 0.038
-
 NAD+ pH 9.0, 37°C Escherichia coli K-12
1.2.1.24 0.047
-
 succinate semialdehyde pH 9.0, 37°C Escherichia coli K-12
1.2.1.79 0.01
-
 succinate semialdehyde pH 9.0, 37°C Escherichia coli K-12
1.2.1.79 0.04
-
 NADP+ pH 9.0, 37°C Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.24 Escherichia coli K-12
-
-
-
1.2.1.79 Escherichia coli K-12
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.24 -15°C, 14 h, 95% residual activity Escherichia coli K-12
1.2.1.24 18°C, 14 h, 93% residual activity Escherichia coli K-12
1.2.1.79 -15C, 14 h, 81% residual activity Escherichia coli K-12
1.2.1.79 18C, 14 h, 78% residual activity Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.24 additional information no substrate: formaldehyde, acetaldehyde, glyoxal, glyoxalate, propanal, glutaraldehyde, benzaldehyde, and anisaldehyde Escherichia coli K-12 ?
-
 ?
1.2.1.24 n-butanal + NAD+ + H2O about 11% of the rate with succinic semialdehyde Escherichia coli K-12 butanoic acid + NADH + 2 H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Escherichia coli K-12 succinate + NADH + 2 H+
-
 ?
1.2.1.79 additional information no substrate: n-butanal, formaldehyde, acetaldehyde, glyoxal, glyoxalate, propanal, glutaraldehyde, benzaldehyde, and anisaldehyde Escherichia coli K-12 ?
-
 ?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli K-12 succinate + NADPH + 2 H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.24 37
-
 assay at Escherichia coli K-12
1.2.1.79 37
-
 assay at Escherichia coli K-12

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.24 18
-
 14 h, 93% residual activity Escherichia coli K-12
1.2.1.24 37
-
 14 h, 92% residual activity Escherichia coli K-12
1.2.1.79 18
-
 14 h, 78% residual activity Escherichia coli K-12
1.2.1.79 37
-
 14 h, 69% residual activity Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.24 9
-
-
 Escherichia coli K-12
1.2.1.79 9
-
-
 Escherichia coli K-12

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.24 8
-
 rapid decrease in stability above Escherichia coli K-12
1.2.1.79 8
-
 rapid decrease in stability above Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.24 NADP+
-
 Escherichia coli K-12

Expression

EC Number Organism Comment Expression
1.2.1.24 Escherichia coli K-12 induced by growth of cells on gamma-aminobutanoate up
1.2.1.79 Escherichia coli K-12 decrease by growth of cells on gamma-aminobutanoate down