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Literature summary extracted from

  • Park, J.H.; Dorrestein, P.C.; Zhai, H.; Kinsland, C.; McLafferty, F.W.; Begley, T.P.
    Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1) (2003), Biochemistry, 42, 12430-12438.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.8.1.10 synthesis synthesis of the thiazole moiety of thiamin from glycine, cysteine, and deoxy-D-xylulose-5-phosphate using overexpressed Bacillus subtilis ThiF, ThiS, ThiO, ThiG, and a NifS-like protein Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.73 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
2.8.1.10 expression in Escherichia coli Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.73 Mg2+ required Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.73 Bacillus subtilis O31619
-
-
2.8.1.10 Bacillus subtilis O31618
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.73 Ni-NTA column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.73 ATP + [sulfur-carrier protein ThiS]
-
 Bacillus subtilis diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
 ?
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] ThiG alone can catalyze the formation of thiazole phosphate Bacillus subtilis 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.73 ThiF
-
 Bacillus subtilis
2.8.1.10 ThiG
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.73 ATP
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.7.7.73 metabolism ThiF participates in the biosynthesis of thiazole phosphate Bacillus subtilis
2.8.1.10 physiological function biochemical function of each of the proteins involved in biosynthesis of thiamin diphosphate is as follws: ThiF catalyzes the adenylation of ThiS, NifS catalyzes the transfer of sulfur from cysteine to the acyl adenylate of ThiS, ThiO catalyzes the oxidation of glycine to the corresponding imine, and ThiG catalyzes the formation of the thiazole phosphate ring Bacillus subtilis