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Literature summary extracted from

  • Sillero, M.A.; de Diego, A.; Tavares, J.E.; Silva, J.A.; Perez-Zuniga, F.J.; Sillero, A.
    Synthesis of ATP derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by T4 RNA ligase, T4 DNA ligase and other ligases (2009), Biochem. Pharmacol., 78, 335-343.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.05
-
 farnesyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.15
-
 (2E,6E)-farnesyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 0.21
-
 geranyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.31
-
 geranyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 0.63
-
 isopentenyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 2.3
-
 isopentenyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 4
-
 dimethylallyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 0.07
-
 (2E,6E)-farnesyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 0.23
-
 geranyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 0.24
-
 (2E,6E)-farnesyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 0.29
-
 geranyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 0.86
-
 dimethylallyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 3.6
-
 isopentenyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 4.9
-
 isopentenyl diphosphate
-
 Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Tequatrovirus T4
-
-
-
6.5.1.3 Tequatrovirus T4
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.3 0.0034
-
 with geranyl diphosphate as substrate Tequatrovirus T4
6.5.1.3 0.0037
-
 with dimethylallyl diphosphate as substrate Tequatrovirus T4
6.5.1.3 0.004
-
 with (2E,6E)-farnesyl diphosphate as substrate Tequatrovirus T4
6.5.1.3 0.0062
-
 with isopentenyl diphosphate as substrate Tequatrovirus T4
6.5.1.3 0.016
-
 with (2E,6E)-farnesyl triphosphate as substrate Tequatrovirus T4
6.5.1.3 0.0166
-
 with geranyl triphosphate as substrate Tequatrovirus T4
6.5.1.3 0.0335
-
 with isopentenyl triphosphate as substrate Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (2E,6E)-farnesyl diphosphate
-
 Tequatrovirus T4 adenosine 5'-((2E,6E)-farnesyl triphosphate) + diphosphate
-
 ?
6.5.1.1 ATP + (2E,6E)-farnesyl triphosphate
-
 Tequatrovirus T4 adenosine 5'-((2E,6E)-farnesyl tetraphosphate) + diphosphate
-
 ?
6.5.1.1 ATP + dimethylallyl diphosphate
-
 Tequatrovirus T4 adenosine 5'-dimethylallyltriphosphate + diphosphate
-
 ?
6.5.1.1 ATP + geranyl diphosphate
-
 Tequatrovirus T4 adenosine 5'-geranyl triphosphate + diphosphate
-
 ?
6.5.1.1 ATP + geranyl triphosphate
-
 Tequatrovirus T4 adenosine 5'-geranyl tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + isopentenyl diphosphate
-
 Tequatrovirus T4 adenosine 5'-isopentenyl triphosphate + diphosphate
-
 ?
6.5.1.1 ATP + isopentenyl triphosphate
-
 Tequatrovirus T4 adenosine 5'-isopentenyl tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + tripolyphosphate
-
 Tequatrovirus T4 adenosine 5'-triphosphate derivative of tripolyphosphate + diphosphate
-
 ?
6.5.1.3 ATP + (2E,6E)-farnesyl diphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 (2E,6E)-farnesyl-triphospho(5')adenosine + diphosphate
-
 ir
6.5.1.3 ATP + (2E,6E)-farnesyl triphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 (2E,6E)-farnesyl-tetraphospho(5')adenosine + diphosphate
-
 ir
6.5.1.3 ATP + dimethylallyl diphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 dimethylallyl-triphospho(5')adenosine + diphosphate
-
 ir
6.5.1.3 ATP + geranyl diphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 geranyl-triphospho(5')adenosine + diphosphate
-
 ir
6.5.1.3 ATP + geranyl triphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 geranyl-tetraphospho(5')adenosine + diphosphate
-
 ir
6.5.1.3 ATP + isopentenyl diphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 adenosine 5'-isopentenyl triphosphate + diphosphate
-
 ir
6.5.1.3 ATP + isopentenyl triphosphate assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP Tequatrovirus T4 isopentenyl-tetraphospho(5')adenosine + diphosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 T4 DNA ligase
-
 Tequatrovirus T4
6.5.1.3 RNA ligase
-
 Tequatrovirus T4
6.5.1.3 T4 RNA ligase
-
 Tequatrovirus T4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.1 0.0008
-
 geranyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0028
-
 farnesyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0316
-
 isopentenyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0319
-
 geranyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0349
-
 (2E,6E)-farnesyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0492
-
 dimethylallyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0729
-
 isopentenyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 0.9
-
 isopentenyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 3.1
-
 dimethylallyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 6.7
-
 isopentenyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 8.6
-
 geranyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 11.6
-
 (2E,6E)-farnesyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 12.1
-
 (2E,6E)-farnesyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 52.2
-
 geranyl triphosphate
-
 Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
-
 Tequatrovirus T4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.5.1.1 0.0038
-
 geranyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0123
-
 dimethylallyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.0138
-
 isopentenyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.056
-
 farnesyl diphosphate
-
 Tequatrovirus T4
6.5.1.1 0.103
-
 geranyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 0.116
-
 isopentenyl triphosphate
-
 Tequatrovirus T4
6.5.1.1 0.232
-
 farnesyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 2.5
-
 geranyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 2.7
-
 dimethylallyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 2.9
-
 (2E,6E)-farnesyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 4.5
-
 isopentenyl diphosphate
-
 Tequatrovirus T4
6.5.1.3 12
-
 geranyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 24
-
 isopentenyl triphosphate
-
 Tequatrovirus T4
6.5.1.3 166
-
 (2E,6E)-farnesyl triphosphate
-
 Tequatrovirus T4