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Literature summary extracted from

  • Scherzinger, D.; Ruch, S.; Kloer, D.P.; Wilde, A.; Al-Babili, S.
    Retinal is formed from apo-carotenoids in Nostoc sp. PCC7120: in vitro characterization of an apo-carotenoid oxygenase (2006), Biochem. J., 398, 361-369.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.75 0.0021
-
 (3R)-3hydroxy-beta-apo-8'-carotenal 20°C, pH 6.8 Nostoc sp. PCC 7120 = FACHB-418
1.13.11.75 0.016
-
 beta-apo-8'-carotenal 20°C, pH 6.8 Nostoc sp. PCC 7120 = FACHB-418
1.13.11.75 0.017
-
 beta-apo-10'-carotenal 20°C, pH 6.8 Nostoc sp. PCC 7120 = FACHB-418
1.13.11.75 0.0245
-
 beta-apo-8'-carotenol 20°C, pH 6.8 Nostoc sp. PCC 7120 = FACHB-418

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.75 membrane monotopic membrane protein Nostoc sp. PCC 7120 = FACHB-418 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.75 Nostoc sp. PCC 7120 = FACHB-418 Q8YPB4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.75 (3R)-3hydroxy-beta-apo-12'-carotenal + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 (3R)-3-hydroxy-retinal + ?
-
 ?
1.13.11.75 (3R)-3hydroxy-beta-apo-8'-carotenal + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 (3R)-3-hydroxy-retinal + apo-8',15'-apo-carotenedial
-
 ?
1.13.11.75 (3R)-3hydroxy-beta-apo-8'-carotenol + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 (3R)-3-hydroxy-retinal + apo-8',15'-apo-carotenedial
-
 ?
1.13.11.75 apo-10'-lycopenal + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 acycloretinal + ?
-
 ?
1.13.11.75 apo-8'-lycopenal + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 acycloretinal + ?
-
 ?
1.13.11.75 apo-8'-lycopenol + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 acycloretinal + ?
-
 ?
1.13.11.75 beta-apo-10'-carotenal + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 retinal + ?
-
 ?
1.13.11.75 beta-apo-8'-carotenal + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 retinal + apo-8',15'-apo-carotenedial
-
 ?
1.13.11.75 beta-apo-8'-carotenol + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 retinal + apo-8',15'-apo-carotenedial
-
 ?
1.13.11.75 additional information enzyme exhibits a wide substrate specificity with respect to chain lengths and functional endgroups, converting beta-apo-carotenals, (3R)-3-hydroxy-beta-apocarotenals and the corresponding alcohols into retinal and (3R)-3-hydroxyretinal, respectively. Cleavage occurs at the C15-C15' double bond. No substrates: beta-apo-4'-carotenal, beta-apo-12'-carotenal Nostoc sp. PCC 7120 = FACHB-418 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.75 ACO
-
 Nostoc sp. PCC 7120 = FACHB-418
1.13.11.75 apo-carotenoid oxygenase
-
 Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.75 20
-
 assay at Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.75 6.8
-
 assay at Nostoc sp. PCC 7120 = FACHB-418