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Literature summary extracted from

  • Zarai, Z.; Bacha, A.B.; Horchani, H.; Bezzine, S.; Zouari, N.; Gargouri, Y.; Mejdoub, H.
    A novel hepatopancreatic phospholipase A2 from Hexaplex trunculus with digestive and toxic activities (2010), Arch. Biochem. Biophys., 494, 121-129.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 additional information slight increase in phospholipase activity during the summer Hexaplex trunculus
3.1.1.4 sodium deoxycholate is required for activity, maximum activity above 4 mM Hexaplex trunculus
3.1.1.4 sodium taurodeoxycholate is required for activity, maximum activity above 4 mM Hexaplex trunculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 EDTA
-
 Hexaplex trunculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ specific activity increases to reach its maximum in the presence of 10 mM Ca2+ using phosphatidylcholine as substrate Hexaplex trunculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 30000
-
 gel filtration Hexaplex trunculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Camelus dromedarius
-
-
-
3.1.1.4 Hexaplex trunculus
-
-
-
3.1.1.4 Struthio camelus
-
-
-
3.1.1.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Camelus dromedarius
3.1.1.4
-
 Struthio camelus
3.1.1.4 by ammonium sulfate and ethanol precipitation and gel filtration, to homogeneity, 250fold. Overall recovery of phospholipase activity is about 82% Hexaplex trunculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 hepatopancreas
-
 Hexaplex trunculus
-
3.1.1.4 pancreas
-
 Sus scrofa
-
3.1.1.4 pancreas
-
 Camelus dromedarius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.72
-
 crude extract, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2 Hexaplex trunculus
3.1.1.4 180
-
 250fold purified enzyme, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2 Hexaplex trunculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-stearoyl-2-oleoyl-3-sn-glycerophosphorylcholine + H2O
-
 Hexaplex trunculus ?
-
 ?
3.1.1.4 egg yolk + H2O
-
 Hexaplex trunculus ?
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Hexaplex trunculus 1-acylglycerophosphocholine + fatty acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 monomer 1 * 30000, SDS-PAGE Hexaplex trunculus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 marine snail digestive phospholipase A2
-
 Hexaplex trunculus
3.1.1.4 mSDPL
-
 Hexaplex trunculus
3.1.1.4 phospholipase A2
-
 Sus scrofa
3.1.1.4 phospholipase A2
-
 Camelus dromedarius
3.1.1.4 phospholipase A2
-
 Struthio camelus
3.1.1.4 phospholipase A2
-
 Hexaplex trunculus
3.1.1.4 PLA2
-
 Sus scrofa
3.1.1.4 PLA2
-
 Camelus dromedarius
3.1.1.4 PLA2
-
 Struthio camelus
3.1.1.4 PLA2
-
 Hexaplex trunculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 50
-
-
 Hexaplex trunculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.4 35 60
-
 Hexaplex trunculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 40
-
 retains more than 80% or 40% of its initial activity after 30 min of incubation at 40 or 50°C, respectively. The enzyme is completely inactivated after incubation at 55°C during 30 min. Presence of substrate increases thermostability Hexaplex trunculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8.5
-
-
 Hexaplex trunculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.4 6 10
-
 Hexaplex trunculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.4 7 9 is highly stable over a broad pH range, maintains over 100% of its maximal activity between pH 7.0 and 9.0 and 60% at pH 10. The enzyme is completely inactivated at pH 4.0 for 30 min Hexaplex trunculus

General Information

EC Number General Information Comment Organism
3.1.1.4 physiological function does not show any hemolysis activity in intact erythrocytes Sus scrofa
3.1.1.4 physiological function does not show any hemolysis activity in intact erythrocytes. Is active at low surface pressure Camelus dromedarius
3.1.1.4 physiological function does not show any hemolysis activity in intact erythrocytes. Is active at low surface pressure Struthio camelus
3.1.1.4 physiological function exhibits hemolytic activity in intact erythrocytes and can penetrate phospholipid monolayers at high surface pressure. May be toxic to mammal cells Hexaplex trunculus