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Literature summary extracted from

  • Zheng, Y.; Xue, Y.; Zhang, Y.; Zhou, C.; Schwaneberg, U.; Ma, Y.
    Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4 (2010), Appl. Microbiol. Biotechnol., 87, 225-233.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression in Escherichia coli Caldanaerobacter subterraneus subsp. tengcongensis MB4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Cu2+
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 EDTA
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 Pb2+
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 Zn2+
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 1.35
-
maltotetraose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 1.63
-
maltopentaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 1.88
-
maltohexaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 1.93
-
maltotriose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 2.21
-
maltoheptaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 2.41
-
isomaltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 13.4
-
maltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ca2+ slight increase in enzyme activtiy Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 Mn2+ slight increase in enzyme activity Caldanaerobacter subterraneus subsp. tengcongensis MB4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 77000
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Caldanaerobacter subterraneus subsp. tengcongensis MB4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 partially purification to 30fold homogeneity by heat treatment and gel filtration chromatography Caldanaerobacter subterraneus subsp. tengcongensis MB4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 80
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 175
-
after purification Caldanaerobacter subterraneus subsp. tengcongensis MB4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 isomaltose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
3.2.1.3 maltoheptaose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
3.2.1.3 maltohexaose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
3.2.1.3 maltopentaose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
3.2.1.3 maltose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
3.2.1.3 maltotetraose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?
3.2.1.3 maltotriose + H2O assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 monomer
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 TtcGA
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 75
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 75
-
no activity loss after incubation at 75°C for 6 h, half life at 80°C 2.6 h Caldanaerobacter subterraneus subsp. tengcongensis MB4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 10.7
-
isomaltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 149
-
maltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 343
-
maltoheptaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 368
-
maltotriose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 459
-
maltohexaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 460
-
maltotetraose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 518
-
maltopentaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5
-
-
Caldanaerobacter subterraneus subsp. tengcongensis MB4

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 5 7 above and below abruptly loss of enzyme activity Caldanaerobacter subterraneus subsp. tengcongensis MB4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.3 4.44
-
isomaltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 11.1
-
maltose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 155
-
maltoheptaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 191
-
maltotriose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 244
-
maltohexaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 318
-
maltopentaose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.3 341
-
maltotetraose pH 5.0, 75°C Caldanaerobacter subterraneus subsp. tengcongensis MB4