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Literature summary extracted from

  • Glück, M.; Lingens, F.
    Heteroxanthinedemethylase, a new enzyme in the degradation of caffeine by Pseudomonas putida (1988), Appl. Microbiol. Biotechnol., 28, 59-62.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.13.128 stabilization can be achieved by addition of either glycerol (20%), bovine serum albumin (10 mg/ml), dithiothreitol (1 mM) or the substrate 7-methylxanthine (1 mM). Storage under nitrogen atmosphere has a stabilizing influence. The addition of caffeine (1 mM), dimethylxanthines (1 mM) and protease inhibitors (pepstatin A, PMSF, 0.1 mM) is without influence on stability Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.128 Ag+ 0.2 mM, complete inhibition Pseudomonas putida
1.14.13.128 Co2+ 0.2 mM, 40% inhibition Pseudomonas putida
1.14.13.128 Cu2+ 0.2 mM, complete inhibition Pseudomonas putida
1.14.13.128 Fe2+ 1 mM, 37% inhibition Pseudomonas putida
1.14.13.128 Hg2+ 1 mM, 98% inhibition Pseudomonas putida
1.14.13.128 Sn2+ 0.2 mM, 17% inhibition Pseudomonas putida
1.14.13.128 Zn2+ 0.2 mM, complete inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.128 0.113
-
 O2 pH and temperature not specified in the publication Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.128 Ca2+ 1 mM, activation to 123% of control Pseudomonas putida
1.14.13.128 Mg2+ 1 mM, activation to 132% of control Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.128 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.128 partial Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.13.128 4°C, pH 6.0, 94% loss of activity after 24 h Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.128 7-methylxanthine + O2 + NADH + H+
-
 Pseudomonas putida xanthine + NAD+ + H2O + formaldehyde
-
 ?
1.14.13.128 7-methylxanthine + O2 + NADPH + H+
-
 Pseudomonas putida xanthine + NADP+ + H2O + formaldehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.128 30 35
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.128 45
-
 1 min, complete inactivation Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.128 7.3
-
-
 Pseudomonas putida