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Literature summary extracted from

  • Chhokar, V.; Sangwan, M.; Beniwal, V.; Nehra, K.; Nehra, K.S.
    Effect of additives on the activity of tannase from Aspergillus awamori MTCC9299 (2010), Appl. Biochem. Biotechnol., 160, 2256-2264.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 Benzene increases activity by 2fold at 60% v/v concentration Aspergillus awamori
3.1.1.20 butanol increases activity by 2fold at 60% v/v concentration Aspergillus awamori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 acetic acid completely inhibits activity Aspergillus awamori
3.1.1.20 acetone gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 55.01% Aspergillus awamori
3.1.1.20 chloroform completely inhibits activity Aspergillus awamori
3.1.1.20 Co2+ at 1 mM inhibits by 71.14% Aspergillus awamori
3.1.1.20 Cu2+ at 1 mM inhibits by 51.21% Aspergillus awamori
3.1.1.20 ethanol inhibits by 48.84% initially, thereafter complete loss in the enzyme activity at 40% and 60% Aspergillus awamori
3.1.1.20 Fe3+ at 1 mM inhibits by 76.89% Aspergillus awamori
3.1.1.20 Isoamylalcohol completely inhibits activity Aspergillus awamori
3.1.1.20 Isopropyl alcohol completely inhibits activity Aspergillus awamori
3.1.1.20 methanol initial inhibitory effect at 20% and 40%, original activity is regained at 60% Aspergillus awamori
3.1.1.20 additional information 1 mM Zn2+ has no effect Aspergillus awamori
3.1.1.20 Toluene gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 28.49% Aspergillus awamori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ at 1 mM stimulates by 16% Aspergillus awamori
3.1.1.20 K+ at 1 mM stimulates by 9% Aspergillus awamori
3.1.1.20 Mg2+ at 1 mM stimulates by 23.9% Aspergillus awamori
3.1.1.20 Mn2+ at 1 mM stimulates by 15.23% Aspergillus awamori
3.1.1.20 Na+ at 1 mM stimulates by 11% Aspergillus awamori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 101000
-
 x * 101000, SDS-PAGE Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus awamori
-
 MTCC9299
-
3.1.1.20 Aspergillus awamori MTCC9299
-
 MTCC9299
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 by ammonium sulfate precipitation followed by ion-exchange chromatography, 19.5folf with 13.5% yield, to homogeneity Aspergillus awamori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 489
-
 crude extract Aspergillus awamori
3.1.1.20 9550
-
 19.5fold purified enzyme Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Aspergillus awamori gallic acid + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus awamori MTCC9299 gallic acid + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 101000, SDS-PAGE Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 30
-
-
 Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5.5
-
-
 Aspergillus awamori