BRENDA - Enzyme Database

Effect of additives on the activity of tannase from Aspergillus awamori MTCC9299

Chhokar, V.; Sangwan, M.; Beniwal, V.; Nehra, K.; Nehra, K.S.; Appl. Biochem. Biotechnol. 160, 2256-2264 (2010)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.20
Benzene
increases activity by 2fold at 60% v/v concentration
Aspergillus awamori
3.1.1.20
butanol
increases activity by 2fold at 60% v/v concentration
Aspergillus awamori
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.20
acetic acid
completely inhibits activity
Aspergillus awamori
3.1.1.20
acetone
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 55.01%
Aspergillus awamori
3.1.1.20
chloroform
completely inhibits activity
Aspergillus awamori
3.1.1.20
Co2+
at 1 mM inhibits by 71.14%
Aspergillus awamori
3.1.1.20
Cu2+
at 1 mM inhibits by 51.21%
Aspergillus awamori
3.1.1.20
ethanol
inhibits by 48.84% initially, thereafter complete loss in the enzyme activity at 40% and 60%
Aspergillus awamori
3.1.1.20
Fe3+
at 1 mM inhibits by 76.89%
Aspergillus awamori
3.1.1.20
Isoamylalcohol
completely inhibits activity
Aspergillus awamori
3.1.1.20
Isopropyl alcohol
completely inhibits activity
Aspergillus awamori
3.1.1.20
methanol
initial inhibitory effect at 20% and 40%, original activity is regained at 60%
Aspergillus awamori
3.1.1.20
additional information
1 mM Zn2+ has no effect
Aspergillus awamori
3.1.1.20
Toluene
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 28.49%
Aspergillus awamori
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.20
Ca2+
at 1 mM stimulates by 16%
Aspergillus awamori
3.1.1.20
K+
at 1 mM stimulates by 9%
Aspergillus awamori
3.1.1.20
Mg2+
at 1 mM stimulates by 23.9%
Aspergillus awamori
3.1.1.20
Mn2+
at 1 mM stimulates by 15.23%
Aspergillus awamori
3.1.1.20
Na+
at 1 mM stimulates by 11%
Aspergillus awamori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.20
101000
-
x * 101000, SDS-PAGE
Aspergillus awamori
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.20
Aspergillus awamori
-
MTCC9299
-
3.1.1.20
Aspergillus awamori MTCC9299
-
MTCC9299
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.20
by ammonium sulfate precipitation followed by ion-exchange chromatography, 19.5folf with 13.5% yield, to homogeneity
Aspergillus awamori
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.20
489
-
crude extract
Aspergillus awamori
3.1.1.20
9550
-
19.5fold purified enzyme
Aspergillus awamori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.20
tannic acid + H2O
-
707202
Aspergillus awamori
gallic acid + D-glucose
-
-
-
?
3.1.1.20
tannic acid + H2O
-
707202
Aspergillus awamori MTCC9299
gallic acid + D-glucose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.20
?
x * 101000, SDS-PAGE
Aspergillus awamori
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.1.20
tannase
-
Aspergillus awamori
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.20
30
-
-
Aspergillus awamori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.20
5.5
-
-
Aspergillus awamori
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.20
Benzene
increases activity by 2fold at 60% v/v concentration
Aspergillus awamori
3.1.1.20
butanol
increases activity by 2fold at 60% v/v concentration
Aspergillus awamori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.20
acetic acid
completely inhibits activity
Aspergillus awamori
3.1.1.20
acetone
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 55.01%
Aspergillus awamori
3.1.1.20
chloroform
completely inhibits activity
Aspergillus awamori
3.1.1.20
Co2+
at 1 mM inhibits by 71.14%
Aspergillus awamori
3.1.1.20
Cu2+
at 1 mM inhibits by 51.21%
Aspergillus awamori
3.1.1.20
ethanol
inhibits by 48.84% initially, thereafter complete loss in the enzyme activity at 40% and 60%
Aspergillus awamori
3.1.1.20
Fe3+
at 1 mM inhibits by 76.89%
Aspergillus awamori
3.1.1.20
Isoamylalcohol
completely inhibits activity
Aspergillus awamori
3.1.1.20
Isopropyl alcohol
completely inhibits activity
Aspergillus awamori
3.1.1.20
methanol
initial inhibitory effect at 20% and 40%, original activity is regained at 60%
Aspergillus awamori
3.1.1.20
additional information
1 mM Zn2+ has no effect
Aspergillus awamori
3.1.1.20
Toluene
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 28.49%
Aspergillus awamori
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.20
Ca2+
at 1 mM stimulates by 16%
Aspergillus awamori
3.1.1.20
K+
at 1 mM stimulates by 9%
Aspergillus awamori
3.1.1.20
Mg2+
at 1 mM stimulates by 23.9%
Aspergillus awamori
3.1.1.20
Mn2+
at 1 mM stimulates by 15.23%
Aspergillus awamori
3.1.1.20
Na+
at 1 mM stimulates by 11%
Aspergillus awamori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.20
101000
-
x * 101000, SDS-PAGE
Aspergillus awamori
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.20
by ammonium sulfate precipitation followed by ion-exchange chromatography, 19.5folf with 13.5% yield, to homogeneity
Aspergillus awamori
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.20
489
-
crude extract
Aspergillus awamori
3.1.1.20
9550
-
19.5fold purified enzyme
Aspergillus awamori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.20
tannic acid + H2O
-
707202
Aspergillus awamori
gallic acid + D-glucose
-
-
-
?
3.1.1.20
tannic acid + H2O
-
707202
Aspergillus awamori MTCC9299
gallic acid + D-glucose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.20
?
x * 101000, SDS-PAGE
Aspergillus awamori
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.20
30
-
-
Aspergillus awamori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.20
5.5
-
-
Aspergillus awamori