Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Geissler, J.F.; Harwood, C.S.; Gibson, J.
    Purification and properties of benzoate-coenzyme A ligase, a Rhodopseudomonas palustris enzyme involved in the anaeriobic degradation of benzoate (1988), J. Bacteriol., 170, 1709-1714.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.25 benzoyl-CoA product inhibition Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.0006 0.002 benzoate
-
Rhodopseudomonas palustris
6.2.1.25 0.002 0.003 ATP
-
Rhodopseudomonas palustris
6.2.1.25 0.09 0.12 reduced CoA
-
Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.25 Mg2+ required Rhodopseudomonas palustris
6.2.1.25 Mn2+ can replace Mg2+ Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 58000
-
HPLC gel filtration Rhodopseudomonas palustris
6.2.1.25 60000
-
1 * 60000, SDS-PAGE Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.25 ATP + benzoate + CoA Rhodopseudomonas palustris enzyme is involved in anaerobic degradation of benzoate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Rhodopseudomonas palustris
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
6.2.1.25 purified enzyme is insensitive to oxygen Rhodopseudomonas palustris

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25
-
Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 25
-
-
Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
6.2.1.25 0°C, 1 mg/ml purified enzyme, half-life is 8 days Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-chlorobenzoate + CoA 10% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + 2-chlorobenzoyl-CoA
-
?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 102% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + 2-fluorobenzoyl-CoA
-
?
6.2.1.25 ATP + 4-fluorobenzoate + CoA 72% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + 4-fluorobenzoyl-CoA
-
?
6.2.1.25 ATP + benzoate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + benzoyl-CoA
-
?
6.2.1.25 ATP + benzoate + CoA enzyme is involved in anaerobic degradation of benzoate Rhodopseudomonas palustris ?
-
?
6.2.1.25 ATP + DELTA1-cyclohexenecarboxylate + CoA 13% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA
-
?
6.2.1.25 ATP + DELTA3-cyclohexenecarboxylate + CoA 54% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA
-
?
6.2.1.25 ATP + nicotinate + CoA 12% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + nicotinyl-CoA
-
?
6.2.1.25 ATP + picolinate + CoA 45% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + picolinyl-CoA
-
?
6.2.1.25 additional information no reaction with GTP Rhodopseudomonas palustris ?
-
?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 monomer 1 * 60000, SDS-PAGE Rhodopseudomonas palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.25 8.4 8.9
-
Rhodopseudomonas palustris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.25 7 8.9 7.0: 10% of maximal activity, 8.0: 75% of maximal activity, 8.4-8.9: maximal activity Rhodopseudomonas palustris