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Literature summary extracted from

  • Han, S.; Chang, J.S.; Griffor, M.
    Structure of the adenylation domain of NAD+-dependent DNA ligase from Staphylococcus aureus (2009), Acta Crystallogr. Sect. F, 65, 1078-1082.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.2 hanging drop vapor diffusion method, using 2.1-2.5 M ammonium sulfate and 0.1 M MES or HEPES pH 6.8-7.5 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.2 75080
-
 calculated from amino acid sequence Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Staphylococcus aureus Q9AIU7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 HisTrap FF column chromatography, hydroxyapatite type I column chromatography, and HiPrep Q column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Staphylococcus aureus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 LigA
-
 Staphylococcus aureus
6.5.1.2 NAD+-dependent DNA ligase
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+
-
 Staphylococcus aureus