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Literature summary extracted from

  • WU, A.; ZHANG, Y.; ZHENG, C.; DAI, Y.; LIU, Y.; ZENG, J.; GU, G.; LIU, J.
    Purification and enzymatic characteristics of cysteine desulfurase, IscS, in Acidithiobacillus ferrooxidans ATCC 23270 (2008), Trans. Nonferrous Met. Soc. China, 18, 1450-1457.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.7 expressed in Escherichia coli BL21(DE3) cells Acidithiobacillus ferrooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.7 N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine up to 90% inhibition at 5 mM Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.7 0.11
-
 L-cysteine in 50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 5 mM dithiothreitol, at 25°C Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.7 46000
-
 SDS-PAGE Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Acidithiobacillus ferrooxidans
-
 strain ATCC 23270
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.7 Hi-Trap column chromatography Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.7 0.0224
-
 crude extract, at 25°C Acidithiobacillus ferrooxidans
2.8.1.7 0.148
-
 enzyme after purification, at 25°C Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine IscS has a high affinity for L-cysteine Acidithiobacillus ferrooxidans L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.7 IscS
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.7 30
-
-
 Acidithiobacillus ferrooxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.1.7 40 55 the enzyme is relatively stable at 40°C with 67% loss of activity, and unstable at temperatures above 55°C Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.7 8 8.5
-
 Acidithiobacillus ferrooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.1.7 7 9
-
 Acidithiobacillus ferrooxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.1.7 5 10 about 45% residual activity at pH 7.5 and 9.5, almost no activity at pH 5.0 and 10.0 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.7 pyridoxal 5'-phosphate
-
 Acidithiobacillus ferrooxidans