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Literature summary extracted from

  • Koutmos, M.; Datta, S.; Pattridge, K.A.; Smith, J.L.; Matthews, R.G.
    Insights into the reactivation of cobalamin-dependent methionine synthase (2009), Proc. Natl. Acad. Sci. USA, 106, 18527-18532.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.13 expressed in Escherichia coli Hms174(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.13 hanging drop vapor diffusion method using 0.2 M potassium nitrate, 18% (w/v) PEG3350, or microbatch vapor diffusion method using 0.2 M potassium nitrate, 20% (w/v) PEG3350, 50 mM HEPES pH 7.5 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.13 Y1139F the mutation lowers the cobalamine reduction potential from -490 mV to -540 mV Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Escherichia coli P13009
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 nickel affinity chromatography using a Hi-Trap and a MonoQ column Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Escherichia coli tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.13 cobalamin-dependent methionine synthase
-
 Escherichia coli
2.1.1.13 MetH
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 vitamin B12
-
 Escherichia coli