Literature summary extracted from
Datta, S.; Koutmos, M.; Pattridge, K.A.; Ludwig, M.L.; Matthews, R.G.
A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor (2008), Proc. Natl. Acad. Sci. USA, 105, 4115-4120.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.13 |
expressed in Escherichia coli Hms174(DE3) cells |
Escherichia coli |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.1.1.13 |
the 65-kDa I690C/G743C MetH fragment is crystallized by the microbatch method, using 0.2 M potassium nitrate and 20% (w/v) PEG3350 |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.1.1.13 |
I690C/G743C |
the mutant is locked in the reactivation conformation and found in both His-on and -off states |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.13 |
Escherichia coli |
P13009 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.1.13 |
Ni-charged HiTrap chelating column chromatography and Mono Q column chromatography |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.13 |
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine |
- |
Escherichia coli |
tetrahydropteroylmonoglutamate + L-methionine |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.1.1.13 |
B12-dependent methionine synthase |
- |
Escherichia coli |
2.1.1.13 |
MetH |
- |
Escherichia coli |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.1.13 |
vitamin B12 |
- |
Escherichia coli |
|