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Literature summary extracted from

  • Datta, S.; Koutmos, M.; Pattridge, K.A.; Ludwig, M.L.; Matthews, R.G.
    A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor (2008), Proc. Natl. Acad. Sci. USA, 105, 4115-4120.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.13 expressed in Escherichia coli Hms174(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.13 the 65-kDa I690C/G743C MetH fragment is crystallized by the microbatch method, using 0.2 M potassium nitrate and 20% (w/v) PEG3350 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.13 I690C/G743C the mutant is locked in the reactivation conformation and found in both His-on and -off states Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Escherichia coli P13009
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 Ni-charged HiTrap chelating column chromatography and Mono Q column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Escherichia coli tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.13 B12-dependent methionine synthase
-
 Escherichia coli
2.1.1.13 MetH
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 vitamin B12
-
 Escherichia coli