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Literature summary extracted from

  • Malojcic, G.; Owen, R.L.; Grimshaw, J.P.; Brozzo, M.S.; Dreher-Teo, H.; Glockshuber, R.
    A structural and biochemical basis for PAPS-independent sulfuryl transfer by aryl sulfotransferase from uropathogenic Escherichia coli (2008), Proc. Natl. Acad. Sci. USA, 105, 19217-19222.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.22 phenol
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.22 0.0445
-
 4-Methylumbelliferyl sulfate at pH 8.0, at 25°C Escherichia coli
2.8.2.22 1.35
-
 phenol at pH 8.0, at 25°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.22 periplasm
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 63765
-
 2 * 63765, freshly purified enzyme, electrospray ionization mass spectrometry Escherichia coli
2.8.2.22 63800
-
 2 * 63800, X-ray crystallography Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.22 additional information Escherichia coli arylsulfate sulfotransferase follows a ping-pong bi-bi reaction mechanism, in which the catalytic residue His-436 undergoes transient sulfurylation ?
-
 ?
2.8.2.22 additional information Escherichia coli CFT073 arylsulfate sulfotransferase follows a ping-pong bi-bi reaction mechanism, in which the catalytic residue His-436 undergoes transient sulfurylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Escherichia coli Q8FDI4
-
-
2.8.2.22 Escherichia coli CFT073 Q8FDI4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 3'-phosphoadenylylsulfate + 4-methylumbelliferol
-
 Escherichia coli adenosine 3',5'-bisphosphate + 4-methylumbelliferyl sulfate
-
 r
2.8.2.22 3'-phosphoadenylylsulfate + 4-methylumbelliferol
-
 Escherichia coli CFT073 adenosine 3',5'-bisphosphate + 4-methylumbelliferyl sulfate
-
 r
2.8.2.22 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Escherichia coli adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 r
2.8.2.22 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Escherichia coli CFT073 adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 r
2.8.2.22 3'-phosphoadenylylsulfate + phenol
-
 Escherichia coli adenosine 3',5'-bisphosphate + phenyl sulfate
-
 r
2.8.2.22 3'-phosphoadenylylsulfate + phenol
-
 Escherichia coli CFT073 adenosine 3',5'-bisphosphate + phenyl sulfate
-
 r
2.8.2.22 additional information arylsulfate sulfotransferase follows a ping-pong bi-bi reaction mechanism, in which the catalytic residue His-436 undergoes transient sulfurylation Escherichia coli ?
-
 ?
2.8.2.22 additional information arylsulfate sulfotransferase follows a ping-pong bi-bi reaction mechanism, in which the catalytic residue His-436 undergoes transient sulfurylation Escherichia coli CFT073 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 homodimer 2 * 63765, freshly purified enzyme, electrospray ionization mass spectrometry Escherichia coli
2.8.2.22 homodimer 2 * 63800, X-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.8.2.22 arylsulfate sulfotransferase
-
 Escherichia coli
2.8.2.22 ASST
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.22 48.6
-
 phenol at pH 8.0, at 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.2.22 adenosine 3',5'-bisphosphate
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.22 0.048
-
 phenol at pH 8.0, at 25°C Escherichia coli
2.8.2.22 1.1
-
 phenol at pH 10.0, at 25°C Escherichia coli