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Literature summary extracted from

  • Caillat, C.; Topalis, D.; Agrofoglio, L.A.; Pochet, S.; Balzarini, J.; Deville-Bonne, D.; Meyer, P.
    Crystal structure of poxvirus thymidylate kinase: an unexpected dimerization has implications for antiviral therapy (2008), Proc. Natl. Acad. Sci. USA, 105, 16900-16905.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.9 TMPK bound to TDP and to brivudin monophosphate, hanging drop vapor diffusion method Vaccinia virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 (E)-5-(2-bromovinyl)-deoxyuridine
-
 Vaccinia virus
2.7.4.9 5-bromo-deoxyuridine
-
 Vaccinia virus
2.7.4.9 5-chloro-deoxyuridine
-
 Vaccinia virus
2.7.4.9 5-fluoro-deoxyuridine
-
 Vaccinia virus
2.7.4.9 5-iodo-deoxyuridine
-
 Vaccinia virus
2.7.4.9 deoxythymidine
-
 Vaccinia virus
2.7.4.9 deoxyuridine
-
 Vaccinia virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.19
-
 (E)-5-(2-bromovinyl)-deoxyuridine monophosphate
-
 Vaccinia virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.9 Mg2+ contains Mg2+ at the active site Vaccinia virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Vaccinia virus P68693
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + (E)-5-(2-bromovinyl)-deoxyuridine monophosphate 5% of the TMP efficiency Vaccinia virus ?
-
 ?
2.7.4.9 ATP + brivudin
-
 Vaccinia virus ?
-
 ?
2.7.4.9 ATP + dGMP 5% of the TMP efficiency Vaccinia virus ?
-
 ?
2.7.4.9 ATP + dUMP
-
 Vaccinia virus ?
-
 ?
2.7.4.9 ATP + TMP
-
 Vaccinia virus ?
-
 ?
2.7.4.9 N-methanocarbathymidine
-
 Vaccinia virus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.9 dimer x-ray crystallography Vaccinia virus

Synonyms

EC Number Synonyms Comment Organism
2.7.4.9 thymidylate kinase
-
 Vaccinia virus
2.7.4.9 TMPK
-
 Vaccinia virus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.9 42.5
-
 the midtemperature of thermal denaturation is 42.5°C Vaccinia virus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.9 0.86
-
 (E)-5-(2-bromovinyl)-deoxyuridine monophosphate
-
 Vaccinia virus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.9 ATP
-
 Vaccinia virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.007
-
 5-bromo-deoxyuridine
-
 Vaccinia virus
2.7.4.9 0.015
-
 5-chloro-deoxyuridine
-
 Vaccinia virus
2.7.4.9 0.023
-
 5-iodo-deoxyuridine
-
 Vaccinia virus
2.7.4.9 0.025
-
 deoxythymidine
-
 Vaccinia virus
2.7.4.9 0.5
-
 (E)-5-(2-bromovinyl)-deoxyuridine
-
 Vaccinia virus
2.7.4.9 0.7
-
 5-fluoro-deoxyuridine
-
 Vaccinia virus
2.7.4.9 1
-
 deoxyuridine
-
 Vaccinia virus