Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Blankenfeldt, W.; Kuzin, A.P.; Skarina, T.; Korniyenko, Y.; Tong, L.; Bayer, P.; Janning, P.; Thomashow, L.S.; Mavrodi, D.V.
    Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens (2004), Proc. Natl. Acad. Sci. USA, 101, 16431-16436.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.17 expression in Escherichia coli Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.17 hanging-drop vapor-diffusion method. Crystals of unliganded PhzF belong to space group P3(2)21 with a a = b = 56 A, and c = 156 A and hold one monomer per asymmetric unit. Complexes of PhzF (with 3-hydroxyanthranilic acid and trans-2,3-dihydro-3-hydroxyanthranilic acid) crystallize in space group P2(1)2(1)2 with cell parameters of a = 93 A, b = 100 A, and c = 57 A and contain one dimer in the asymmetric unit Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.17 42000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.17 (5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate Pseudomonas fluorescens i.e. trans-2,3-dihydro-3-hydroxyanthranilic acid. The enzyme is involved in phenazine synthesis (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate i.e. 2,3-dihydro-3-hydroxy-anthranilate ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.17 Pseudomonas fluorescens Q51792
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.17
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.17 (5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate i.e. trans-2,3-dihydro-3-hydroxyanthranilic acid. The enzyme is involved in phenazine synthesis Pseudomonas fluorescens (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate i.e. 2,3-dihydro-3-hydroxy-anthranilate ?
5.3.3.17 (5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate charactierzation of the product by NMR spectrometry. The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an isomerase using the conserved glutamate45 to abstract a proton from C3 of the substrate. The proposed mechanism involves proton abstraction from C3 of trans-2,3-dihydro-3-hydroxyanthranilate by Glu45, followed by rearrangement of the double bonds and reprotonation at C1. The function of E45 as a proton shuttle is supported by results from NMR spectroscopy Pseudomonas fluorescens (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.17 dimer deduced from crystal structure. The crystal structure clarifies that the enzyme is a dimer in an up/up configuration with the two active sites facing each other. The dimer in the open form, generates by crystal symmetry (sulfate complex), interacts mainly through the alpha1-helices and the beta16-strands in the N-terminal domains. The C-terminal domains are not in direct contact but are relatively close Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
5.3.3.17 phzF
-
 Pseudomonas fluorescens