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Literature summary extracted from

  • Ernst, C.M.; Staubitz, P.; Mishra, N.N.; Yang, S.J.; Hornig, G.; Kalbacher, H.; Bayer, A.S.; Kraus, D.; Peschel, A.
    The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion (2009), PLoS Pathog., 5, e1000660.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.2.3 medicine MprF could be a target for new anti-virulence drugs Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.3 into the vector pET28 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.3 D546A MprF(-8) mutant, replacement results in slightly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 D731A MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 E624A MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 E685A MprF(-8) mutant, replacement results in strongly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 K547A MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 K621A MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 K806A MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus
2.3.2.3 MprF(-10) mutant, truncated protein consisting of residues 363-840 Staphylococcus aureus
2.3.2.3 MprF(-12) mutant, truncated protein consisting of residues 437-840 Staphylococcus aureus
2.3.2.3 MprF(-14) mutant, truncated protein consisting of residues 510-840 Staphylococcus aureus
2.3.2.3 MprF(-2) mutant, truncated protein consisting of residues 84-840 Staphylococcus aureus
2.3.2.3 MprF(-4) mutant, truncated protein consisting of residues 157-840 Staphylococcus aureus
2.3.2.3 MprF(-6) mutant, truncated protein consisting of residues 219-840 Staphylococcus aureus
2.3.2.3 MprF(-8) mutant, truncated protein consisting of residues 274-840 Staphylococcus aureus
2.3.2.3 MprF(-C) mutant, truncated protein consisting of residues 1-586 Staphylococcus aureus
2.3.2.3 R734A MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.3 membrane
-
Staphylococcus aureus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.3 39000
-
theoretical, MprF(-14) mutant, truncated protein consisting of residues 510-840 Staphylococcus aureus
2.3.2.3 48000
-
theoretical, MprF(-12) mutant, truncated protein consisting of residues 437-840 Staphylococcus aureus
2.3.2.3 56000
-
theoretical, MprF(-10) mutant, truncated protein consisting of residues 363-840 Staphylococcus aureus
2.3.2.3 67000
-
theoretical, MprF(-8) mutant, truncated protein consisting of residues 274-840 Staphylococcus aureus
2.3.2.3 67000
-
theoretical, MprF(-C) mutant, truncated protein consisting of residues 1-586 Staphylococcus aureus
2.3.2.3 75000
-
theoretical, MprF(-6) mutant, truncated protein consisting of residues 219-840 Staphylococcus aureus
2.3.2.3 80000
-
theoretical, MprF(-4) mutant, truncated protein consisting of residues 157-840 Staphylococcus aureus
2.3.2.3 88000
-
theoretical, MprF(-2) mutant, truncated protein consisting of residues 84-840 Staphylococcus aureus
2.3.2.3 97000
-
theoretical, MprF wild-type Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol Staphylococcus aureus
-
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.3 Staphylococcus aureus Q2G2M2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol
-
Staphylococcus aureus tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.3 lysyltransferase
-
Staphylococcus aureus
2.3.2.3 MprF protein
-
Staphylococcus aureus
2.3.2.3 multiple peptide resistance factor protein
-
Staphylococcus aureus