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Literature summary extracted from

  • Kuchenreuther, J.M.; Stapleton, J.A.; Swartz, J.R.
    Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation (2009), PLoS ONE, 4, e7565.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.99.6 3,4-dihydroxy-L-phenylalanine substitutes for tyrosine to stimulate in vitro HydA1 activation, improves HydA1 maturation 4fold Chlamydomonas reinhardtii
1.12.99.6 additional information guanosine triphosphate does not improve hydrogenase maturation Chlamydomonas reinhardtii
1.12.99.6 additional information tyrosine enhances in vitro maturation of HydA1 hydrogenase. Tyrosine has the most significant effect on overall HydA1 activities. Cysteine enhances in vitro maturation of HydA1 hydrogenase. Cysteine has the most significant effect on early HydA1 activities Chlamydomonas reinhardtii
1.12.99.6 S-adenosyl methionine exogenous S-adenosyl methionine stimulates in vitro hydrogenase activation, but does not individually enhance hydrogenase activation. 2 mM increases hydrogenase activity 4fold Chlamydomonas reinhardtii
1.12.99.6 Sulfide 1 mM increases hydrogenase activity 4fold, is not sufficient to increase hydrogenase activities without S-adenosyl methionine and the standard 20 L-amino acids Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 heterologously expressed in Escherichia coli BL21(DE3) from vector pY71 N-His6-shydA1* Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe2+ 1 mM increases hydrogenase activity 4fold, is not sufficient to increase hydrogenase activities without S-adenosyl methionine and the standard 20 L-amino acids Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 48400
-
 x * 48400, SDS-PAGE, N-His6-HydA1 Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 by immobilized metal-affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + oxidized methyl viologen
-
 Chlamydomonas reinhardtii H+ + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 ? x * 48400, SDS-PAGE, N-His6-HydA1 Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 HydA1
-
 Chlamydomonas reinhardtii
1.12.99.6 [FeFe]-hydrogenase
-
 Chlamydomonas reinhardtii