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Literature summary extracted from

  • Muroi, A.; Ishihara, A.; Tanaka, C.; Ishizuka, A.; Takabayashi, J.; Miyoshi, H.; Nishioka, T.
    Accumulation of hydroxycinnamic acid amides induced by pathogen infection and identification of agmatine coumaroyltransferase in Arabidopsis thaliana (2009), Planta, 230, 517-527.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.64 PCR-amplification, expression in Escherichia coli BL21(DE3) Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.64 agmatine the presence of this substrate inhibits the enzyme reaction with putrescine Arabidopsis thaliana
2.3.1.64 enterokinase the removal of the thioredoxin domain by enterokinase decreases the enzyme activity but does not alter the relative amounts of products Arabidopsis thaliana
2.3.1.64 additional information putrescine or feruloyl-CoA do not inhibit the enzyme reaction with agmantine and p-coumaroyl-CoA Arabidopsis thaliana
2.3.1.64 p-Coumaroyl-CoA the presence of this substrate inhibits the enzyme reaction with feruloyl-CoA Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.64 70000
-
SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.64 feruloyl-CoA + agmatine Arabidopsis thaliana 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors CoA + feruloyl agmatine
-
?
2.3.1.64 feruloyl-CoA + putrescine Arabidopsis thaliana 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors CoA + feruloylputrescine
-
?
2.3.1.64 p-coumaroyl-CoA + agmatine Arabidopsis thaliana 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors CoA + p-coumaroylagmatine
-
?
2.3.1.64 p-coumaroyl-CoA + putrescine Arabidopsis thaliana 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors CoA + p-coumaroylputrescine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.64 Arabidopsis thaliana
-
wild-type and mutants, infection of rosette leaves with the fungus Alternaria brassicicola strain MAFF237450
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.64 frozen leaves are homogenized in 100 mM Tris buffer, pH 7.5, containing 10 mM 2-mercaptoethanol and 5 mM DTT, centrifugation, supernatant applied to Sephadex G-50 column, fluent used for enzyme assays, or recombinant Escherichia coli cells are harvested and resuspended in 100 mM Tris buffer, pH 7.5, containing 1 mM EDTA, 50 mM NaCl, 10% glycerol, lysed with lysozyme, sonicated, centrifuged, supernatant used for enzyme assays and SDS-PAGE Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.64 leaf cut off rosette leaves infected with the fungus Alternaria brassicicola Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.64 0.00000003
-
with substrates p-coumaroyl-CoA + agmatine + putrescine 0.0018 nmol coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.000000085
-
with substrates feruloyl-CoA + putrescine 0.0051 nmol feruloylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.000000933
-
with substrates p-coumaroyl-CoA + putrescine 0.056 nmol p-coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.00002
-
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 1.2 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.00004
-
with substrates feruloyl-CoA + agmatine 2.4 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.000112
-
with substrates p-coumaroyl-CoA + agmatine 6.7 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.000123
-
with substrates p-coumaroyl-CoA + agmatine + putrescine 7.4 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana
2.3.1.64 0.000187
-
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 11.2 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.64 feruloyl-CoA + agmatine 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors Arabidopsis thaliana CoA + feruloyl agmatine
-
?
2.3.1.64 feruloyl-CoA + putrescine 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors Arabidopsis thaliana CoA + feruloylputrescine
-
?
2.3.1.64 p-coumaroyl-CoA + agmatine 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors Arabidopsis thaliana CoA + p-coumaroylagmatine
-
?
2.3.1.64 p-coumaroyl-CoA + putrescine 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors Arabidopsis thaliana CoA + p-coumaroylputrescine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.64 agmatine coumaroyltransferase
-
Arabidopsis thaliana
2.3.1.64 At5g61160 protein
-
Arabidopsis thaliana
2.3.1.64 AtACT
-
Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.3.1.64 Arabidopsis thaliana upon infection with Alternaria brassicicola enzyme activity in leaves increases markedly from near zero to almost 20 nmol/mg protein/h within 24 h coinciding with an increase in gene transcripts and in the synthesis products, that accumulate in the area of the lesion, slow decrease beyond 24 h up

General Information

EC Number General Information Comment Organism
2.3.1.64 metabolism last step in the synthesis of hydroxycinnamic acid amides in plants (anti-pathogenic metabolites), in Arabidopsis thaliana infected with Alternaria brassicicola p-coumaroylagmatine, feruloylagmatine, p-coumaroylputrescine, and feruloylputrescine are identified as anti-fungal agents in vitro and in vivo Arabidopsis thaliana