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Literature summary extracted from

  • Wang, Z.; Zhu, Y.; Wang, L.; Liu, X.; Liu, Y.; Phillips, J.; Deng, X.
    A WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase (BhGolS1) promoter (2009), Planta, 230, 1155-1166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.123 coding region of BhGolS1 is inframe cloned into pGEX-4T-1 downstream to GST coding region and transformed into Escherichia coli BL21(DE3) and BL21 codon plus cells, generation of transgenic tobacco plants, constructs are introduced into Agrobacterium strain LBA4404 by electroporation and transformed into tobacco via a leaf disc method Dorcoceras hygrometricum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.123 MnCl2 5 mM are included in assay medium Dorcoceras hygrometricum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol Dorcoceras hygrometricum
-
 UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.123 Dorcoceras hygrometricum B6UYJ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.123 the soluble protein is separated by SDS-PAGE to check the molecular weight of the recombinant protein, which is then purified using Glutathione Sepharose TM 4B Dorcoceras hygrometricum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.123 leaf
-
 Dorcoceras hygrometricum
-
2.4.1.123 seedling
-
 Dorcoceras hygrometricum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.123 additional information
-
 BhGolS1 activity enhances drought tolerance in transgenic tobacco, a higher survival rate of transgenic plants after rewatering (84.8% for S10-1 tobacco line, 72.6% for S22-5 tobacco line, 61.5% for Rd15-6 tobacco line), compared to wild type (38.4%) is observed Dorcoceras hygrometricum
2.4.1.123 3.8
-
 purified BhGolS1-GST fusion protein is able to catalyze galactinol synthesis, 50 mM Hepes buffer, pH 7.0, 2 mM dithiothreitol, 5 mM MnCl2 and 0.5-1 mg purified enzyme protein Dorcoceras hygrometricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol
-
 Dorcoceras hygrometricum UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.123 BhGolS1
-
 Dorcoceras hygrometricum
2.4.1.123 galactinol synthase
-
 Dorcoceras hygrometricum
2.4.1.123 GOLS
-
 Dorcoceras hygrometricum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.123 30
-
 assay at Dorcoceras hygrometricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.123 7
-
 assay at Dorcoceras hygrometricum

Expression

EC Number Organism Comment Expression
2.4.1.123 Dorcoceras hygrometricum a WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase promoter, GolS genes are induced by a variety of stresses in both stress-sensitive and tolerant-plant species, mRNA and protein accumulate in leaves dehydrated for 2-48 h, and disappear after rehydration, BhGolS1 is rapidly induced by abscisic acid at the mRNA level after 0.5 h and at the protein level after 8 h up

General Information

EC Number General Information Comment Organism
2.4.1.123 physiological function first committed step of the raffinose family oligosaccharides biosynthetic pathway Dorcoceras hygrometricum