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Literature summary extracted from

  • Degenhardt, J.; Gershenzon, J.
    Demonstration and characterization of (E)-nerolidol synthase from maize: a herbivore-inducible terpene synthase participating in (3E)-4,8-dimethyl-1,3,7-nonatriene biosynthesis (2000), Planta, 210, 815-822.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.48 EDTA 1 mM, activity is fully restored by the addition of Mg2+ to a saturating concentration of 1 mM. Mn2+ is about half as effective as Mg2+ at 1 mM Zea mays
4.2.3.48 Mn2+ activates at 1 mM, inhibits at higher concentrations Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.48 0.0038
-
 (2E,6E)-farnesyl diphosphate
-
 Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.48 Co2+ nerolidol synthase is completely inactive in the absence of added divalent metal ion. Mg2+ is most effective. Co2+ shows 11% of the activity compared to Mg2+ Zea mays
4.2.3.48 Cu2+ nerolidol synthase is completely inactive in the absence of added divalent metal ion. Mg2+ is most effective. Cu2+ shows 12% of the activity compared to Mg2+ Zea mays
4.2.3.48 Mg2+ nerolidol synthase is completely inactive in the absence of added divalent metal ion. Mg2+ is most effective. Other divalent cations are less effective in supporting catalysis. Metal ions in order of edcreasing efficiency: Mg2+, Mn2+, Co2+, Cu2+, Ni2+, Zn2+. KM-value for Mg2+ is 0.125 mM Zea mays
4.2.3.48 Mn2+ nerolidol synthase is completely inactive in the absence of added divalent metal ion. Mn2+ is half as effective as Mg2+ at 1 mM, inhibits at higher concentrations Zea mays
4.2.3.48 Ni2+ nerolidol synthase is completely inactive in the absence of added divalent metal ion. Mg2+ is most effective. Ni2+ shows 17% of the activity compared to Mg2+ Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.48 50000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.48 (2E,6E)-farnesyl diphosphate + H2O Zea mays biosynthesis of the C11 homoterpene (3E)-4,8-dimethyl-1,3,7-nonatriene (3S,6E)-nerolidol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.48 Zea mays
-
 cv. Delprim
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.48 leaf leaves fed upon by Spodoptera littoralis Zea mays
-

Storage Stability

EC Number Storage Stability Organism
4.2.3.48 -80°C, stable for 1 week Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.48 (2E,6E)-farnesyl diphosphate + H2O biosynthesis of the C11 homoterpene (3E)-4,8-dimethyl-1,3,7-nonatriene Zea mays (3S,6E)-nerolidol + diphosphate
-
 ?
4.2.3.48 (2E,6E)-farnesyl diphosphate + H2O the enzymer does not form linalool or other metabolites from geranyl diphosphate Zea mays (3S,6E)-nerolidol + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.48 6.8
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.48 6.4 7.2 50% of maximal activity at pH 6.4 and pH 7.2 Zea mays