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Literature summary extracted from

  • Georgelis, N.; Shaw, J.R.; Hannah, L.C.
    Phylogenetic analysis of ADP-glucose pyrophosphorylase subunits reveals a role of subunit interfaces in the allosteric properties of the enzyme (2009), Plant Physiol., 151, 67-77.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate 3-PGA Solanum tuberosum
2.7.7.27 3-phosphoglycerate 3-PGA Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 for expression in Escherichia coli AC70R1-504 cells Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 A508S large subunit mutation Zea mays
2.7.7.27 C114A large subunit mutation Zea mays
2.7.7.27 C382F large subunit mutation Zea mays
2.7.7.27 C424V large subunit mutation Zea mays
2.7.7.27 D368S large subunit mutation Zea mays
2.7.7.27 E438Q large subunit mutation Zea mays
2.7.7.27 H149S large subunit mutation Zea mays
2.7.7.27 M172T large subunit mutation Zea mays
2.7.7.27 P372A large subunit mutation Zea mays
2.7.7.27 Q213H large subunit mutation Zea mays
2.7.7.27 S163F large subunit mutation Zea mays
2.7.7.27 T361C large subunit mutation Zea mays
2.7.7.27 V227R large subunit mutation Zea mays
2.7.7.27 V502T large subunit mutation Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 inorganic phosphate
-
 Solanum tuberosum
2.7.7.27 phosphate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.05
-
 alpha-D-glucose 1-phosphate mutant BT2/E438Q Zea mays
2.7.7.27 0.05
-
 alpha-D-glucose 1-phosphate mutant BT2/P372A Zea mays
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate mutant BT2/A508S Zea mays
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate mutant BT2/M172T Zea mays
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate mutant BT2/T361C Zea mays
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate mutant BT2/V227R Zea mays
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate mutant BT2/V502T Zea mays
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate mutant BT2/C114A Zea mays
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate mutant BT2/D368S Zea mays
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate mutant BT2/H149S Zea mays
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate mutant BT2/Q213H Zea mays
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate wild type BT2/SH2 Zea mays
2.7.7.27 0.08
-
 alpha-D-glucose 1-phosphate mutant BT2/C382F Zea mays
2.7.7.27 0.09
-
 ATP mutant BT2/A508S Zea mays
2.7.7.27 0.09
-
 alpha-D-glucose 1-phosphate mutant BT2/C424V Zea mays
2.7.7.27 0.09
-
 ATP mutant BT2/M172T Zea mays
2.7.7.27 0.09
-
 alpha-D-glucose 1-phosphate mutant BT2/S163F Zea mays
2.7.7.27 0.11
-
 ATP mutant BT2/D368S Zea mays
2.7.7.27 0.12
-
 ATP wild type BT2/SH2 Zea mays
2.7.7.27 0.13
-
 ATP mutant BT2/E438Q Zea mays
2.7.7.27 0.13
-
 ATP mutant BT2/T361C Zea mays
2.7.7.27 0.14
-
 ATP mutant BT2/C114A Zea mays
2.7.7.27 0.14
-
 ATP mutant BT2/C424V Zea mays
2.7.7.27 0.14
-
 ATP mutant BT2/V227R Zea mays
2.7.7.27 0.15
-
 ATP mutant BT2/C382F Zea mays
2.7.7.27 0.15
-
 ATP mutant BT2/V502T Zea mays
2.7.7.27 0.19
-
 ATP mutant BT2/H149S Zea mays
2.7.7.27 0.19
-
 ATP mutant BT2/P372A Zea mays
2.7.7.27 0.21
-
 ATP mutant BT2/Q213H Zea mays
2.7.7.27 0.42
-
 ATP mutant BT2/S163F Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Solanum tuberosum
-
 diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Zea mays
-
 diphosphate + ADP-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum tuberosum Q00081 fragment
-
2.7.7.27 Zea mays P55241
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 by protamine sulfate and ammonium sulfate fractionation, followed by anion-exchange and affinity chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Zea mays
-
2.7.7.27 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer consists of two small and two large subunits Solanum tuberosum
2.7.7.27 heterotetramer consists of two small and two large subunits, BT2/SH2 Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 ADP-glucose pyrophosphorylase
-
 Solanum tuberosum
2.7.7.27 ADP-glucose pyrophosphorylase
-
 Zea mays
2.7.7.27 AGPase
-
 Solanum tuberosum
2.7.7.27 AGPase
-
 Zea mays
2.7.7.27 BT2 maize AGPase endosperm small subunit Zea mays
2.7.7.27 SH2 maize AGPase endosperm large subunit Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.27 37
-
 activity assay Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.27 16.17
-
 alpha-D-glucose 1-phosphate mutant BT2/V227R Zea mays
2.7.7.27 23.34
-
 alpha-D-glucose 1-phosphate mutant BT2/D368S Zea mays
2.7.7.27 29.36
-
 alpha-D-glucose 1-phosphate mutant BT2/Q213H Zea mays
2.7.7.27 32.87
-
 alpha-D-glucose 1-phosphate mutant BT2/E438Q Zea mays
2.7.7.27 34.65
-
 alpha-D-glucose 1-phosphate mutant BT2/S163F Zea mays
2.7.7.27 35.21
-
 alpha-D-glucose 1-phosphate mutant BT2/H149S Zea mays
2.7.7.27 35.77
-
 alpha-D-glucose 1-phosphate mutant BT2/A508S Zea mays
2.7.7.27 37.21
-
 alpha-D-glucose 1-phosphate mutant BT2/C114A Zea mays
2.7.7.27 38.12
-
 alpha-D-glucose 1-phosphate mutant BT2/T361C Zea mays
2.7.7.27 38.58
-
 alpha-D-glucose 1-phosphate mutant BT2/M172T Zea mays
2.7.7.27 39.17
-
 alpha-D-glucose 1-phosphate wild type BT2/SH2 Zea mays
2.7.7.27 40.75
-
 alpha-D-glucose 1-phosphate mutant BT2/C382F Zea mays
2.7.7.27 41.91
-
 alpha-D-glucose 1-phosphate mutant BT2/V502T Zea mays
2.7.7.27 42.32
-
 alpha-D-glucose 1-phosphate mutant BT2/P372A Zea mays
2.7.7.27 59.86
-
 alpha-D-glucose 1-phosphate mutant BT2/C424V Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 7.4
-
 activity assay Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.27 ATP
-
 Solanum tuberosum
2.7.7.27 ATP
-
 Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 1.83
-
 phosphate mutant BT2/S163F, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 2.28
-
 phosphate mutant BT2/C382F, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 3.21
-
 phosphate mutant BT2/Q213H, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 3.72
-
 phosphate mutant BT2/P372A, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 3.96
-
 phosphate mutant BT2/H149S, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 4.26
-
 phosphate mutant BT2/D368S, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 5.34
-
 phosphate mutant BT2/T361C, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 13.23
-
 phosphate mutant BT2/C114A, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 14.5
-
 phosphate mutant BT2/V227R, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 15.22
-
 phosphate mutant BT2/E438Q, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 16.8
-
 phosphate wild type BT2/SH2, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 17.61
-
 phosphate mutant BT2/M172T, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 17.93
-
 phosphate mutant BT2/V502T, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 18.36
-
 phosphate mutant BT2/C424V, 15 mM 3-phosphoglycerate Zea mays
2.7.7.27 20.43
-
 phosphate mutant BT2/A508S, 15 mM 3-phosphoglycerate Zea mays

General Information

EC Number General Information Comment Organism
2.7.7.27 physiological function ADP-glucose pyrophosphorylase catalyzes the rate-limiting step in starch biosynthesis Solanum tuberosum
2.7.7.27 physiological function ADP-glucose pyrophosphorylase catalyzes the rate-limiting step in starch biosynthesis Zea mays