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Literature summary extracted from

  • Akashi, T.; Sasaki, K.; Aoki, T.; Ayabe, S.; Yazaki, K.
    Molecular cloning and characterization of a cDNA for pterocarpan 4-dimethylallyltransferase catalyzing the key prenylation step in the biosynthesis of glyceollin, a soybean phytoalexin (2009), Plant Physiol., 149, 683-693.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.36 expressed using recombinant yeast microsomes Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.36 0.068
-
 (6aS,11aS)-3,6a,9-trihydroxypterocarpan 30°C Glycine max
2.5.1.36 0.15
-
 dimethylallyl diphosphate 30°C Glycine max
2.5.1.36 45
-
 (6aS,11aS)-3,6a,9-trihydroxypterocarpan using microsomes of cultured soybean cells Glycine max
2.5.1.36 68
-
 (6aS,11aS)-3,6a,9-trihydroxypterocarpan using recombinant yeast microsomes Glycine max
2.5.1.36 150
-
 dimethylallyl diphosphate using recombinant yeast microsomes Glycine max
2.5.1.36 180
-
 dimethylallyl diphosphate using microsomes of cultured soybean cells Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.36 plastid sequence contains a plastid targeting signal Glycine max 9536
-
2.5.1.36 plastid protein sequence is led by a plastid targeting signal sequence Glycine max 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.36 Co2+ 50% activity Glycine max
2.5.1.36 Mg2+ 100% activity Glycine max
2.5.1.36 Mg2+ requirement for divalent cations, with Mg2+ being more effective than Mn2+ Glycine max
2.5.1.36 Mn2+ 68% activity Glycine max
2.5.1.36 Mn2+ requirement for divalent cations, with Mg2+ being more effective than Mn2+ Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.36 Glycine max B9A1Q4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.5.1.36 proteolytic modification sequence contains a plastid targeting signal Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.36 seedling
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.36 2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
-
 Glycine max 2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
-
 ?
2.5.1.36 dimethylallyl diphosphate + (-)-maackiain poor substrate Glycine max diphosphate + ?
-
 ?
2.5.1.36 dimethylallyl diphosphate + (6aS,11aS)-3,6a,9-trihydroxypterocarpan
-
 Glycine max diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
-
 ?
2.5.1.36 additional information no substrate: genistein and daidzein Glycine max ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.36 (6aS,11aS)-3,9,6a-trihydroxypterocarpan [(2)-glycinol] 4-dimethylallyltransferase
-
 Glycine max
2.5.1.36 G4DT
-
 Glycine max
2.5.1.36 pterocarpan 4-dimethylallyltransferase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.36 30
-
 assay Glycine max

Expression

EC Number Organism Comment Expression
2.5.1.36 Glycine max expression is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cell culture up
2.5.1.36 Glycine max expression of the G4DT gene is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cultured soybean cells up